Overview

  • Product name
    Rabbit Anti-Human IgA H&L (FITC)
    See all IgA secondary antibodies
  • Target species
    Human
  • Specificity
    Antibodies were raised in rabbit to human IgA
  • Tested applications
    Suitable for: ELISA, Dot blotmore details
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    50% glycerol, 0.1 M NaCl, 0.01 M sodium phosphate at pH 7.5.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    From the crude polyclonal the crossreactive antibodies were extracted by incubation with Sepharose-bound human IgG and IgM. Specific antibodies were absorpted by incubation with Sepharose-bound human IgA. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human IgA a minimum of 65% protein bound.
  • Conjugation notes
    The purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate.
  • Clonality
    Polyclonal
  • General notes
    F/P ratio is 4-6 mol Fluorescein per 1 mol of rabbit IgA. There is low background fluorescence in normal tissue in contrast to cases of autoimmune desease where IgA is depostited.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Dot blot
  • Application notes
    Recommended dilution as secondary antibody in ELISA is 1:1000.
    Recommended dilution as secondary antibody in dot-blotting is 1:100.
  • References for Rabbit Anti-Human IgA H&L (FITC) (ab8509)

    ab8509 has not yet been referenced specifically in any publications.

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