50% glycerol, 0.1 M NaCl, 0.01 M sodium phosphate at pH 7.5.
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Immunogen affinity purified
From the crude polyclonal the crossreactive antibodies were extracted by incubation with Sepharose-bound human IgM and IgG.
Specific antibodies were absorpted by incubation with Sepharose-bound human IgA.
Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis.
After repeated binding with immobilized human IgA a minimum of 65% protein bound.
The purified polyclonal was conjugated to horseradish peroxidase according to the periodate method followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate.