Overview

  • Product nameRabbit Anti-Human IgA H&L (HRP)
    See all IgA secondary antibodies
  • Target speciesHuman
  • SpecificityAntibodies were raised in rabbit to human IgA
  • Tested applicationsSuitable for: Dot blot, ELISAmore details
  • ConjugationHRP

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage buffer50% glycerol, 0.1 M NaCl, 0.01 M sodium phosphate at pH 7.5.
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesFrom the crude polyclonal the crossreactive antibodies were extracted by incubation with Sepharose-bound human IgM and IgG. Specific antibodies were absorpted by incubation with Sepharose-bound human IgA. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human IgA a minimum of 65% protein bound.
  • Conjugation notesThe purified polyclonal was conjugated to horseradish peroxidase according to the periodate method followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate.
  • ClonalityPolyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot 1/100.
ELISA 1/1000.

References for Rabbit Anti-Human IgA H&L (HRP) (ab8510)

ab8510 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"