Overview

  • Product nameRabbit Anti-Human IgG H&L
    See all IgG secondary antibodies
  • Target speciesHuman
  • SpecificityNo crossreaction with human IgM and IgA in ELISA No crossreaction with albumin fraction of human serum. No crossreaction with albumin fraction of other animal species.
  • Tested applicationsSuitable for: Dot blot, Indirect ELISAmore details
  • Immunogen

    Human IgG.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, 100mM Sodium chloride, 10mM Sodium phosphate, pH 7.5
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesFrom the crude polyclonal the crossreactive antibodies were extracted by incubation with Sepharose-bound human IgA and IgM. Specific antibodies were absorbed by incubation with Sepharose-bound human IgG. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human IgG a minimum of 65% protein bound.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab81048 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot 1/100.
Indirect ELISA 1/1000.

References for Rabbit Anti-Human IgG H&L (ab81048)

ab81048 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab81048.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"