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Product name Rabbit Anti-Human IgG H&L See all IgG secondary antibodies
Target species Human
Specificity No crossreaction with human IgM and IgA in ELISA
No crossreaction with albumin fraction of human serum.
No crossreaction with albumin fraction of other animal species.
Tested applications Suitable for:
Dot blot, Indirect ELISA more details
Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer Preservative: None
Constituents: 50% Glycerol, 100mM Sodium chloride, 10mM Sodium phosphate, pH 7.5
Concentration information loading...
Purity Immunogen affinity purified
Purification notes From the crude polyclonal the crossreactive antibodies were extracted by incubation with Sepharose-bound human IgA and IgM. Specific antibodies were absorbed by incubation with Sepharose-bound human IgG. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human IgG a minimum of 65% protein bound.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Rabbit Anti-Human IgG H&L (ab81048)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"