Rabbit Anti-Human IgG H&L (Alkaline Phosphatase) (ab6760)

Overview

  • Product nameRabbit Anti-Human IgG H&L (Alkaline Phosphatase)
    See all IgG secondary antibodies
  • Target speciesHuman
  • Tested applicationsSuitable for: Immunomicroscopy, WB, ICC/IF, IHC-Fr, Dot blot, IHC-P, ELISAmore details
  • Immunogen

    human IgG whole molecule

  • ConjugationAlkaline Phosphatase

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferpH: 8.00
    Preservative: 0.1% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.788% Tris HCl, 0.0014% Zinc chloride, 0.0095% Magnesium chloride, 50% Glycerol
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads.
  • Conjugation notesAlkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
  • ClonalityPolyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunomicroscopy
WB
ICC/IF
IHC-Fr
Dot blot
IHC-P
ELISA
  • Application notessuitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency
  • References for Rabbit Anti-Human IgG H&L (Alkaline Phosphatase) (ab6760)

    This product has been referenced in:
    • Iversen R  et al. Transglutaminase 2-specific autoantibodies in celiac disease target clustered, N-terminal epitopes not displayed on the surface of cells. J Immunol 190:5981-91 (2013). Read more (PubMed: 23690478) »
    • Kap YS  et al. Fast Progression of Recombinant Human Myelin/Oligodendrocyte Glycoprotein (MOG)-Induced Experimental Autoimmune Encephalomyelitis in Marmosets Is Associated with the Activation of MOG34-56-Specific Cytotoxic T Cells. J Immunol 180:1326-37 (2008). ELISA . Read more (PubMed: 18209026) »

    See all 3 Publications for this product

    Product Wall

    ab6760 is produced against polyclonal whole human IgG purified from serum, and the kappa:lambda ratio of IgG normal human serum is reported to be ~2:1. ab6760 is not tested specifically against kappa or lambda light chains, but it is expected to rec...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"