Rabbit Anti-Human IgG H&L (Alkaline Phosphatase) preadsorbed (ab7161)

Overview

  • Product name
    Rabbit Anti-Human IgG H&L (Alkaline Phosphatase) preadsorbed
    See all IgG secondary antibodies
  • Target species
    Human
  • Tested applications
    Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IFmore details
  • Minimal
    cross-reactivity

    Mouse more details
  • Immunogen

    human IgG whole molecule

  • Conjugation
    Alkaline Phosphatase

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.60
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 50% Glycerol, 0.001% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads.
  • Conjugation notes
    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7161 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot
ELISA
IHC-P
IHC-Fr
Immunomicroscopy
WB
ICC/IF
  • Application notes
    suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency
  • References

    ab7161 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Thank you for your inquiry.
    The lab came back to me with the following answer regarding the cross-reactivity:
    "This antibody is not tested against other immunoglobulins. This antibody reacts with both heavy and light chains of IgG, and cross ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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