Rabbit Anti-Human IgG H&L (Biotin) (ab97158)
Key features and details
- Rabbit Anti-Human IgG H&L (Biotin)
- Conjugation: Biotin
- Host species: Rabbit
- Isotype: IgG
- Suitable for: IHC-P, ICC/IF, ELISA, WB
Overview
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Product name
Rabbit Anti-Human IgG H&L (Biotin)
See all IgG secondary antibodies -
Host species
Rabbit -
Target species
Human -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgG and with light chains common to other Human immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. -
Tested applications
Suitable for: IHC-P, ICC/IF, ELISA, WBmore details -
Conjugation
Biotin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells. -
Conjugation notes
Biotin/ antibody protein ratio is 4:1 -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97158 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/500 - 1/5000.
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ICC/IF |
1/200 - 1/1000.
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ELISA |
1/20000 - 1/400000. (Primary).
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|
WB |
1/20000 - 1/400000. (Chemiluminescent).
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Notes |
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IHC-P
1/500 - 1/5000. |
ICC/IF
1/200 - 1/1000. |
ELISA
1/20000 - 1/400000. (Primary). |
WB
1/20000 - 1/400000. (Chemiluminescent). |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (5)
ab97158 has been referenced in 5 publications.
- Klingler J et al. Immune profiles to distinguish hospitalized versus ambulatory COVID-19 cases in older patients. iScience 25:105608 (2022). PubMed: 36406863
- Klingler J et al. Role of Immunoglobulin M and A Antibodies in the Neutralization of Severe Acute Respiratory Syndrome Coronavirus 2. J Infect Dis 223:957-970 (2021). PubMed: 33367897
- Weiss S et al. A High Through-put Assay for Circulating Antibodies Directed against the S Protein of Severe Acute Respiratory Syndrome Corona virus 2. medRxiv N/A:N/A (2020). PubMed: 32511609
- Weiss S et al. A High-Throughput Assay for Circulating Antibodies Directed Against the S Protein of Severe Acute Respiratory Syndrome Coronavirus 2. J Infect Dis 222:1629-1634 (2020). PubMed: 32860510
- Liao F et al. Targeting of nonlipidated, aggregated apoE with antibodies inhibits amyloid accumulation. J Clin Invest 128:2144-2155 (2018). PubMed: 29600961