Rabbit Anti-Human IgG H&L (Biotin) preadsorbed (ab7159)
Key features and details
- Rabbit Anti-Human IgG H&L (Biotin) preadsorbed
- Conjugation: Biotin
- Host species: Rabbit
- Suitable for: ICC/IF, Immunomicroscopy, WB, Dot blot, ELISA, IHC-Fr, IHC-P
Related conjugates and formulations
Overview
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Product name
Rabbit Anti-Human IgG H&L (Biotin) preadsorbed
See all IgG secondary antibodies -
Host species
Rabbit -
Target species
Human -
Tested applications
Suitable for: ICC/IF, Immunomicroscopy, WB, Dot blot, ELISA, IHC-Fr, IHC-Pmore details -
Minimal
cross-reactivity
Mouse more details -
Immunogen
human IgG whole molecule
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Conjugation
Biotin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.5
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.164% Sodium phosphate, 0.88% Sodium chloride, 1% BSA -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Conjugation notes
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule -
Clonality
Polyclonal -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7159 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | ||
Immunomicroscopy | ||
WB | ||
Dot blot | ||
ELISA | ||
IHC-Fr | ||
IHC-P |
Notes |
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Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab7159 has been referenced in 7 publications.
- Glass LJ et al. Brain antibodies in the cortex and blood of people with schizophrenia and controls. Transl Psychiatry 7:e1192 (2017). IHC-P ; Human . PubMed: 28786974
- Hammar O et al. Autoantibodies and gastrointestinal symptoms in infertile women in relation to in vitro fertilization. BMC Pregnancy Childbirth 13:201 (2013). ELISA . PubMed: 24191889
- Pendleton H et al. Antibodies against gonadotropin-releasing hormone in patients with posterior laryngitis. Drug Target Insights 7:1-8 (2013). PubMed: 23400339
- Roth B & Ohlsson B Gastrointestinal symptoms and psychological well-being in patients with microscopic colitis. Scand J Gastroenterol 48:27-34 (2013). PubMed: 23148737
- Berntorp K et al. Antibodies against gonadotropin-releasing hormone (GnRH) in patients with diabetes mellitus is associated with lower body weight and autonomic neuropathy. BMC Res Notes 6:329 (2013). PubMed: 23958111
- Hammar O et al. Depletion of enteric gonadotropin-releasing hormone is found in a few patients suffering from severe gastrointestinal dysmotility. Scand J Gastroenterol 47:1165-73 (2012). PubMed: 22835010
- Ohlsson B et al. Patients with irritable bowel syndrome and dysmotility express antibodies against gonadotropin-releasing hormone in serum. Neurogastroenterol Motil 23:1000-6, e459 (2011). PubMed: 21714833