Rabbit Anti-Human IgG H&L (Biotin) preadsorbed (ab7159)

Overview

  • Product nameRabbit Anti-Human IgG H&L (Biotin) preadsorbed
    See all IgG secondary antibodies
  • Target speciesHuman
  • Tested applicationsSuitable for: ICC/IF, Immunomicroscopy, WB, Dot blot, ELISA, IHC-Fr, IHC-Pmore details
  • Minimal
    cross-reactivity

    Mouse more details
  • Immunogen

    human IgG whole molecule

  • ConjugationBiotin

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.60
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.164% Sodium phosphate, 0.88% Sodium chloride, 1% BSA
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notesBiotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule
  • ClonalityPolyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Immunomicroscopy
WB
Dot blot
ELISA
IHC-Fr
IHC-P
  • Application notesimmunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency
  • References for Rabbit Anti-Human IgG H&L (Biotin) preadsorbed (ab7159)

    This product has been referenced in:
    • Hammar O  et al. Autoantibodies and gastrointestinal symptoms in infertile women in relation to in vitro fertilization. BMC Pregnancy Childbirth 13:201 (2013). ELISA . Read more (PubMed: 24191889) »
    • Berntorp K  et al. Antibodies against gonadotropin-releasing hormone (GnRH) in patients with diabetes mellitus is associated with lower body weight and autonomic neuropathy. BMC Res Notes 6:329 (2013). Read more (PubMed: 23958111) »

    See all 2 Publications for this product

    Product Wall

    Thank you for your enquiry. This product has been pre-adsorbed against mouse serum proteins only and therefore will show low cross reactivity with mouse.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"