Rabbit Anti-Human IgG H&L (FITC) (ab6755)

Overview

  • Product nameRabbit Anti-Human IgG H&L (FITC)
    See all IgG secondary antibodies
  • Target speciesHuman
  • Tested applicationsSuitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-Fr, ELISAmore details
  • Immunogen

    human whole molecule

  • ConjugationFITC. Ex: 493nm, Em: 528nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferpH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notesDiaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG
  • ClonalityPolyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6755 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunomicroscopy 1/1000 - 1/5000.
Flow Cyt 1/500 - 1/2500.
ICC/IF 1/1000 - 1/5000.
IHC-Fr Use at an assay dependent concentration.
ELISA 1/10000 - 1/50000.

References for Rabbit Anti-Human IgG H&L (FITC) (ab6755)

ab6755 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your inquiry regarding ab6755. This secondary antibody is not designed for ELISA but it could be used for ELISA if the customer has suitable detection system. It could also be used for ELISA with an enzyme linked "tertiary" reagent s...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"