Rabbit Anti-Human IgG H&L (FITC) (ab6755)
Key features and details
- Rabbit Anti-Human IgG H&L (FITC)
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Rabbit
- Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-Fr, ELISA
Related conjugates and formulations
Overview
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Product name
Rabbit Anti-Human IgG H&L (FITC)
See all IgG secondary antibodies -
Host species
Rabbit -
Target species
Human -
Tested applications
Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-Fr, ELISAmore details -
Immunogen
human whole molecule
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Conjugation
FITC. Ex: 493nm, Em: 528nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Tripotassium orthophosphate -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Conjugation notes
Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG -
Clonality
Polyclonal -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6755 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Immunomicroscopy |
1/1000 - 1/5000.
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Flow Cyt |
1/500 - 1/2500.
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|
ICC/IF |
1/1000 - 1/5000.
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|
IHC-Fr |
Use at an assay dependent concentration.
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ELISA |
1/10000 - 1/50000.
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Notes |
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Immunomicroscopy
1/1000 - 1/5000. |
Flow Cyt
1/500 - 1/2500. |
ICC/IF
1/1000 - 1/5000. |
IHC-Fr
Use at an assay dependent concentration. |
ELISA
1/10000 - 1/50000. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab6755 has been referenced in 2 publications.
- Long D et al. Lidocaine inhibits the proliferation and migration of endometrial cancer cells, and promotes apoptosis by inducing autophagy. Oncol Lett 24:347 (2022). PubMed: 36072001
- Jiang Y et al. Long noncoding RNA IRAIN acts as tumor suppressor via miR-125b in multiple myeloma. Oncol Lett 18:6787-6794 (2019). PubMed: 31788123