Overview

  • Product name
    Rabbit Anti-Human IgG H&L (FITC)
    See all IgG secondary antibodies
  • Target species
    Human
  • Specificity
    No cross reactivity with albumine fractions of human serum. No cross reactivity with albumine fractions of animal species.
  • Tested applications
    Suitable for: IHC-Frmore details
  • Immunogen

    Human IgG

  • Conjugation
    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: None
    Constituents: 50% Glycerol, 0.1M Sodium chloride, 0.01M Sodium phosphate, pH 7.5
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    From the crude polyclonal the crossreactive antibodies were extracted by incubation with Sepharose-bound human IgA and IgM. Specific antibodies were absorpted by incubation with Sepharose-bound human IgG. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human IgG a minimum of 65% protein bound.
  • Conjugation notes
    The purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtation and ion-exchange chromatography to clear unbound conjugate.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes


    Low background fluorescence of normal tissue in contrast to cases of autoimmune desease, depostited immunoglobulin IgG.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab81190 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/40.

References

ab81190 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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