Overview

  • Product nameRabbit Anti-Human IgG H&L (FITC)
    See all IgG secondary antibodies
  • Target speciesHuman
  • SpecificityNo cross reactivity with albumine fractions of human serum. No cross reactivity with albumine fractions of animal species.
  • Tested applicationsSuitable for: IHC-Frmore details
  • Immunogen

    Human IgG

  • ConjugationFITC. Ex: 493nm, Em: 528nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, 0.1M Sodium chloride, 0.01M Sodium phosphate, pH 7.5
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesFrom the crude polyclonal the crossreactive antibodies were extracted by incubation with Sepharose-bound human IgA and IgM. Specific antibodies were absorpted by incubation with Sepharose-bound human IgG. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human IgG a minimum of 65% protein bound.
  • Conjugation notesThe purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtation and ion-exchange chromatography to clear unbound conjugate.
  • ClonalityPolyclonal
  • IsotypeIgG
  • General notes


    Low background fluorescence of normal tissue in contrast to cases of autoimmune desease, depostited immunoglobulin IgG.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab81190 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/40.

References for Rabbit Anti-Human IgG H&L (FITC) (ab81190)

ab81190 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"