Rabbit Anti-Human IgG H&L (FITC) preadsorbed (ab7156)
Key features and details
- Rabbit Anti-Human IgG H&L (FITC) preadsorbed
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Rabbit
- Suitable for: IHC-P, IHC-Fr, ICC/IF, Immunomicroscopy, Flow Cyt, ELISA
Overview
-
Product name
Rabbit Anti-Human IgG H&L (FITC) preadsorbed
See all IgG secondary antibodies -
Host species
Rabbit -
Target species
Human -
Tested applications
Suitable for: IHC-P, IHC-Fr, ICC/IF, Immunomicroscopy, Flow Cyt, ELISAmore details -
Minimal
cross-reactivity
Mouse more details -
Immunogen
human whole molecule
-
Conjugation
FITC. Ex: 493nm, Em: 528nm
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Tripotassium orthophosphate -
Concentration information loading...
-
Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Conjugation notes
Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG -
Clonality
Polyclonal -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7156 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use at an assay dependent concentration.
|
|
IHC-Fr |
Use at an assay dependent concentration.
|
|
ICC/IF |
1/1000 - 1/5000.
|
|
Immunomicroscopy |
Use at an assay dependent concentration.
|
|
Flow Cyt |
Use at an assay dependent dilution.
|
|
ELISA |
1/10000 - 1/50000.
|
Notes |
---|
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
ICC/IF
1/1000 - 1/5000. |
Immunomicroscopy
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent dilution. |
ELISA
1/10000 - 1/50000. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab7156 has not yet been referenced specifically in any publications.