Rabbit Anti-Human IgG H&L preadsorbed (ab98568)

Overview

  • Product nameRabbit Anti-Human IgG H&L preadsorbed
    See all IgG secondary antibodies
  • Description
    Rabbit polyclonal Secondary Antibody to Human IgG - H&L, pre-adsorbed
  • Target speciesHuman
  • Tested applicationsSuitable for: ICC, IHC-P, ELISA, WBmore details
  • Minimal
    cross-reactivity

    Mouse, Rat more details

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab98568 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/2000.
ELISA 1/100 - 1/30000. (Coating ELISA: 1/100 - 1/500, Primary ELISA: 1/1000 - 1/30000).
WB 1/1000 - 1/30000. (Colorimetric: 1/1000 - 1/10000, Chemiluminescent: 1/1000 - 1/30000).

References for Rabbit Anti-Human IgG H&L preadsorbed (ab98568)

ab98568 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Immunocytochemistry/ Immunofluorescence
Positive control LP-1 and negative control MY5 (multiple myeloma) cell lines immersion-fixed in 4% PFA, permeablized, blocked in 10% donkey serum/PBS 1 hour and incubated with 1/2000 ab98568 in 10% donkey serum/PBS overnight at 4oC. Washed 3X 5 minutes PBS and incubated with 1/2000 Alexa 488 donkey anti-rabbit F(ab2)" secondary antibody (Jackson). Washed 5X 5 minutes PBS and mounted in DAPI/Vectashield. Images acquired on Zeiss AxioImager FITC channel at 100ms exposure.
Username

Ms. Natalie Erdmann

Verified customer

Submitted Sep 21 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"