Overview

  • Product name
    Rabbit Anti-Human IgG H&L (Texas Red ®)
    See all IgG secondary antibodies
  • Target species
    Human
  • Tested applications
    Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ChIP, ELISAmore details
  • Immunogen

    human IgG whole molecule

  • Conjugation
    Texas Red ®. Ex: 596nm, Em: 620nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Texas RedTM Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 4.9 moles Texas Redä per mole of Rabbit IgG
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6757 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunomicroscopy
Flow Cyt
ICC/IF
IHC-P
IHC-Fr
ChIP
ELISA
  • Application notes
    suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency
    ChIP: Use at a concentration of 6 µg/ml (from PubMed: 17355974).
  • References

    This product has been referenced in:
    • Makino Y  et al. Transcriptional up-regulation of inhibitory PAS domain protein gene expression by hypoxia-inducible factor 1 (HIF-1): a negative feedback regulatory circuit in HIF-1-mediated signaling in hypoxic cells. J Biol Chem 282:14073-82 (2007). Read more (PubMed: 17355974) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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