This product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency
ChIP: Use at a concentration of 6 µg/ml (from PubMed: 17355974).
This product has been referenced in:
Makino Y et al. Transcriptional up-regulation of inhibitory PAS domain protein gene expression by hypoxia-inducible factor 1 (HIF-1): a negative feedback regulatory circuit in HIF-1-mediated signaling in hypoxic cells. J Biol Chem282:14073-82 (2007).
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