Overview

  • Product name
    Rabbit Anti-Human IgG+IgM+IgA H&L (FITC)
    See all IgG + IgM + IgA secondary antibodies
  • Target species
    Human
  • Specificity
    Antibodies were raised in rabbit to human IgG, IgM and IgA
  • Tested applications
    Suitable for: ICC/IFmore details
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    50% glycerol, 0.1 M NaCl, 0.01 M sodium phosphate at pH 7.5.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Specific antibodies were absorpted by incubation with Sepharose-bound human immuno-globulins. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human immuno-globulins a minimum of 65% protein bound.
  • Conjugation notes
    The purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate.
  • Clonality
    Polyclonal
  • General notes
    F/P ratio is 4-6 mol Fluorescein per 1 mol of rabbit Ig. There is low background fluorescence in normal tissue in contrast to cases of autoimmune desease where Ig's are depostited.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.

References for Rabbit Anti-Human IgG+IgM+IgA H&L (FITC) (ab8512)

This product has been referenced in:
  • Pisa D  et al. Evolution of antibody response and fungal antigens in the serum of a patient infected with Candida famata. J Med Microbiol 56:571-8 (2007). ICC/IF . Read more (PubMed: 17446276) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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