Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer50% glycerol, 0.1 M NaCl, 0.01 M sodium phosphate at pH 7.5.
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesFrom the crude polyclonal the cross reactive antibodies were extracted by incubation with Sepharose-bound human IgA and IgG. Specific antibodies were absorpted by incubation with Sepharose-bound human IgM. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human IgM a minimum of 65% protein bound
  • ClonalityPolyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Dot blot
  • Application notesRecommended dilution as secondary antibody in ELISA is 1:1000.
    Recommended dilution as secondary antibody in dot-blotting is 1:100.
  • References for Rabbit Anti-Human IgM H&L (ab8505)

    ab8505 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"