Overview

  • Product name
    Rabbit Anti-Human IgM H&L (FITC)
    See all IgM secondary antibodies
  • Target species
    Human
  • Specificity
    Antibodies were raised in rabbit to human IgM
  • Tested applications
    Suitable for: ICC/IFmore details
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    50% glycerol, 0.1 M NaCl, 0.01 M sodium phosphate at pH 7.5.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    From the crude polyclonal the crossreactive antibodies were extracted by incubation with Sepharose-bound human IgA and IgG. Specific antibodies were absorpted by incubation with Sepharose-bound human IgM. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human IgM a minimum of 65% protein bound.
  • Conjugation notes
    The purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate.
  • Clonality
    Polyclonal
  • General notes
    F/P ratio is 4-6 mol Fluorescein per 1 mol of goat IgM. There is low background fluorescence in normal tissue in contrast to cases of autoimmune desease where IgM is depostited.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
  • Application notes
    For immunohistochemical staining of human tissue sections a 1:40 dilution is recommended.
  • References for Rabbit Anti-Human IgM H&L (FITC) (ab8506)

    ab8506 has not yet been referenced specifically in any publications.

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