Overview

  • Product nameRabbit Anti-Human IgM H&L (FITC)
    See all IgM secondary antibodies
  • Target speciesHuman
  • SpecificityAntibodies were raised in rabbit to human IgM
  • Tested applicationsSuitable for: ICC/IFmore details
  • ConjugationFITC. Ex: 493nm, Em: 528nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage buffer50% glycerol, 0.1 M NaCl, 0.01 M sodium phosphate at pH 7.5.
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesFrom the crude polyclonal the crossreactive antibodies were extracted by incubation with Sepharose-bound human IgA and IgG. Specific antibodies were absorpted by incubation with Sepharose-bound human IgM. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human IgM a minimum of 65% protein bound.
  • Conjugation notesThe purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate.
  • ClonalityPolyclonal
  • General notesF/P ratio is 4-6 mol Fluorescein per 1 mol of goat IgM. There is low background fluorescence in normal tissue in contrast to cases of autoimmune desease where IgM is depostited.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
  • Application notesFor immunohistochemical staining of human tissue sections a 1:40 dilution is recommended.
  • References for Rabbit Anti-Human IgM H&L (FITC) (ab8506)

    ab8506 has not yet been referenced specifically in any publications.

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