Rabbit Anti-Human IgM H&L (FITC) (ab8506)
Key features and details
- Rabbit Anti-Human IgM H&L (FITC)
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Rabbit
- Suitable for: ICC/IF
Related conjugates and formulations
Overview
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Product name
Rabbit Anti-Human IgM H&L (FITC)
See all IgM secondary antibodies -
Host species
Rabbit -
Target species
Human -
Tested applications
Suitable for: ICC/IFmore details -
Conjugation
FITC. Ex: 493nm, Em: 528nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.50
Constituents: 0.164% Sodium phosphate, 50% Glycerol (glycerin, glycerine), 0.58% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
From the crude polyclonal the crossreactive antibodies were extracted by incubation with Sepharose-bound human IgA and IgG. Specific antibodies were absorpted by incubation with Sepharose-bound human IgM. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human IgM a minimum of 65% protein bound. -
Conjugation notes
The purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate. -
Clonality
Polyclonal -
General notes
F/P ratio is 4-6 mol Fluorescein per 1 mol of goat IgM. There is low background fluorescence in normal tissue in contrast to cases of autoimmune desease where IgM is depostited. -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8506 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Notes |
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Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab8506 has been referenced in 1 publication.
- Takegaki J et al. Influence of shortened recovery between resistance exercise sessions on muscle-hypertrophic effect in rat skeletal muscle. Physiol Rep 7:e14155 (2019). PubMed: 31250976