Rabbit anti-IgG Control  - ChIP Grade (ab46540)

Overview

  • Product nameRabbit anti-IgG Control  - ChIP Grade
  • Specificityrecognizes mouse IgG heavy and light chain
  • Tested applicationsELISA, WB, ChIPmore details
  • Immunogen

    Native, mouse whole IgG.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis product was purified by affinity chromatography using mouse IgG-agarose.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas
  • RelevanceIgG is the most abundant class of antibodies found in blood serum and lymph. It is active against bacteria, fungi, viruses, and foreign particles. Immunoglobulin G antibodies trigger action of the complement system.
  • Cellular localizationSecreted
  • Alternative names
    • Constant region of heavy chain of IgG1
    • G1m marker
    • Gm marker
    • Ig gamma 1 chain C region
    • Immunoglobin heavy constant gamma 1
    • Immunoglobulin gamma 1
    see all
  • Cellular localizationSecreted

Applications

Our Abpromise guarantee covers the use of ab46540 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000 - 1/100000.
WB 1/2000 - 1/10000.

This product is suitable for IPPT, Immunodiffusion and most immunological methods requiring lot-to-lot consistency, high titre and specificity.

ChIP Use at an assay dependent concentration.

Protocols

References for Rabbit anti-IgG Control  - ChIP Grade (ab46540)

This product has been referenced in:
  • Yu J  et al. Reduced connexin 43 in eutopic endometrium and cultured endometrial stromal cells from subjects with endometriosis. Mol Hum Reprod 20:260-70 (2014). Read more (PubMed: 24270393) »
  • Biancolella M  et al. Identification and characterization of functional risk variants for colorectal cancer mapping to chromosome 11q23.1. Hum Mol Genet 23:2198-209 (2014). Read more (PubMed: 24256810) »

See all 44 Publications for this product

Product Wall

Thank you for contacting us.


First, I'd like to point out some resources on our website which may help:


http://www.abcam.com/index.html?rid=310


A Beginner's Guide to ChIP - Abcam


As we dis...

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Merci beaucoup pour votre appel de ce matin.

Je suis désolée de devoir vous confirmer que nous devons corriger et informer les clients du produit ab46540, car, grâce à vous, j'ai vérifié et j'ai trouv&...

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Thank you for your inquiry.

Indeed we do have also polyclonal control fractions of mouse or rat IgGs.

For the mouse IgG, we do have the IgG fraction ab37355 http://www.abcam.com/index.html?datasheet=37355 (or use the following: http...

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Thank you for your enquiry.

I have confirmed the purification details of the antibodies you listed and have updated the datasheets where necessary:

ab3609 (Aurora B)
Protein G purified

ab51841 (TATA binding protein...

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Thank you for your inquiry.
I can confim that the antibody was affinity-purified against the antigen itself (bound to agarose).
Protein A or G was not used.
I hope this information is helpful. please do not hesitate to contact me with an...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"