Our Abpromise guarantee covers the use of ab27478 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|ChIP||Use at an assay dependent concentration. PubMed: 20412779|
|IP||Use at an assay dependent concentration. PubMed: 25078649|
|ICC/IF||Use at an assay dependent concentration. PubMed: 17456778|
|IHC-P||1/200. PubMed: 23406280|
Positive immunostaining in brown color, counterstaining with haematoxylin (400× magnification). Only nuclear staining was considered positive. Hes1 is expressed in stage Sertoli and spermatogonia cells (B) using rabbit anti-Hes 1 (ab71559, diluted 1:100). Hes5 is expressed in Sertoli cells and elongated spermatids (D) using rabbit anti-Hes5 (ab25374, diluted 1:100). Control was done with rabbit IgG (A, C) (ab27478). Arrow heads point to spermatogonia cells. Arrows point to Sertoli cells. Tailed arrows point to elongated spermatids.