Rabbit IgG, polyclonal - Isotype Control (ab37415)

Overview

  • Product name
    Rabbit IgG, polyclonal - Isotype Control
    See all Rabbit isotype controls
  • Specificity
    This antibody has been selected to be an isotype control as it has no known specificity. Please note that the rabbit IgG Fc region may bind nonspecifically to human tissue. Rabbit IgG is an isotype control used to estimate the non-specific binding of target primary antibodies due to Fc binding or other protein-protein interactions.
  • Tested applications
    Suitable for: IHC-P, ELISA, Flow Cyt, ChIP/Chipmore details

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 8.20
    Constituent: 100% Borate buffered saline
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Protein A-purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas
  • Alternative names
    • rabbit isotype control

Applications

Our Abpromise guarantee covers the use of ab37415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 24961933
ELISA Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.
ChIP/Chip Use at an assay dependent concentration. PubMed: 20673990

Rabbit IgG, polyclonal - Isotype Control images

  • Histogram showing the fluorescence signal for ADRB2-FITC in PBMCs compared to an Isotype control.

    PBMCs were generated from freshly-drawn blood from healthy human donors. After staining with antibodies against ADRB2 (ab36956) or an isotype control (ab37415), cells were incubated with FITC-labeled secondary anti-rabbit-IgG antibody, and ADRB2 expression was assessed by flow cytometry.

  • ChIP-qPCR for H3K36me2 on the BTF3 locus in NTKO and TKO cells. Methylation enrichment was tested 5 kb upstream of the TSS (5′) and at the TSS (right). ab37415 was used as the isotype contol.

References for Rabbit IgG, polyclonal - Isotype Control (ab37415)

This product has been referenced in:
  • Wang XQ & Dostie J Reciprocal regulation of chromatin state and architecture by HOTAIRM1 contributes to temporal collinear HOXA gene activation. Nucleic Acids Res 45:1091-1104 (2017). ChIP ; Human . Read more (PubMed: 28180285) »
  • Ratovitski EA Tumor Protein (TP)-p53 Members as Regulators of Autophagy in Tumor Cells upon Marine Drug Exposure. Mar Drugs 14:N/A (2016). Read more (PubMed: 27537898) »

See all 33 Publications for this product

Product Wall

The recommended isotype control for use with ab86386 Rabbit polyclonal to HOXB2 is ab37415 Rabbit IgG- ChIP Grade .

Thank you for contacting us.

ab37415 has been tested in flow cytometry as Isotype control.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbi...

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Thank you for your enquiry.

I can confirm that ab134080Anti-CD30 antibody is sold as tissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentrat...

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Thank you for calling Abcam earlier today.

Please find attached the Abcam protocols book that we discussed on the telephone.

Also we do have a few ChiP kits in our catalogue that may be of interest to you:

http://www.abcam.co...

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Merci beaucoup pour votre appel de ce matin.

Je suis désolée de devoir vous confirmer que nous devons corriger et informer les clients du produit ab46540, car, grâce à vous, j'ai vérifié et j'ai trouv&...

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Thank you for your reply.

The only other phospho-smad3 (S423+S425) we have are:

ab11825 - http://www.abcam.com/Smad3-phospho-S423-S425-antibody-ab118825.html

ab52903 - http://www.abcam.com/Smad3-phospho-S423-S425-antibody...

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Thank you very much for contacting Abcam.

The isotype control that I would recommend for your flow cytometry experiments is:

http://www.abcam.com/Rabbit-IgG-ChIP-Grade-ab37415.html



Below is the infromation about...

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Thank you for your inquiry.

The best way to find isotype controls for an antibody is to start at our homepage www.abcam.com. Go to the yellow bar at the top of the page and move the mouse to “Products,” then click on “Iso...

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Thank you for contacting us. Please check ab37415: http://www.abcam.com/Rabbit-IgG-ChIP-Grade-ab37415.html This antibody does not have BSA and sodium azide in it. For ab27472 and and27478 the conc. of azide is very low so theoreticall...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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