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Product Name
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Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (AP), pre-adsorbed
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Product type
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Secondary antibodies
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Description
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Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (AP), pre-adsorbed
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Immunogen
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Rabbit IgG whole molecule
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Tested applications
(see key)
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Dot, ELISA, IHC-Fr, IHC-P, WB
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Application notes
(see key)
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Recommended dilutions
Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency
Please refer to the relevant conjugation for dilutions specific for this product:
Conjugate |
ELISA |
Blotting |
Flow Cytometry |
IHC |
Fluorescein |
nd |
nd |
50 - 200 |
200 - 1,000 |
Rhodamine, Texas RedTM |
nd |
nd |
50 - 200 |
200 - 1,000 |
R-Phycoerythrin |
nd |
nd |
100 - 200 |
nd |
Cyanines |
nd |
nd |
400 |
100 - 1,000 |
Biotin |
5,000 - 20,000 |
3,000 - 10,000 |
nd |
100 - 1,000 |
Peroxidase |
4,000 - 10,000 |
2,000 - 5,000 |
nd |
200 - 2,000 |
Alkaline Phosphatase |
1,000 - 2,000 |
500 - 1,000 |
nd |
100 - 1,000 |
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Research areas
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Secondary antibodies >> anti-Rabbit >> IgG >> Enzyme >> Alkaline Phos
Immunology >> Immunoglobulins >> Heavy Chain >> IgG
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Alternative names Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (AP), pre-adsorbed (ab7084) |
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Raised in
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Donkey
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Clonality
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Polyclonal
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Purity
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IgG fraction
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Conjugation notes
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This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
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Storage buffer
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0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA Material safety datasheet (MSDS) for this product:
Sodium Phosphate MSDS
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Purification notes
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Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
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Form
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Liquid
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Conjugation
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Alkaline Phosphatase
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C. Do not freeze.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (AP), pre-adsorbed is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to IgG
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