Recombinant Rabbit monoclonal [H19-5] Anti-Human kappa light chain (ab125919)
Key features and details
- Rabbit monoclonal [H19-5] Anti-Human kappa light chain
- Host species: Rabbit
- Isotype: IgG
- Suitable for: WB, ELISA
Related conjugates and formulations
Overview
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Product name
Rabbit monoclonal [H19-5] Anti-Human kappa light chain
See all Kappa light chain secondary antibodies -
Host species
Rabbit -
Target species
Human -
Tested applications
Suitable for: WB, ELISAmore details -
Immunogen
Other Immunogen Type corresponding to Human Kappa light chain.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 99% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
H19-5 -
Isotype
IgG -
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
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Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab125919 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/5000.
For unpurified use at 1/1000 - 1/10000. |
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ELISA |
Use a concentration of 1 µg/ml.
For unpurified use at 1/1000 - 1/10000. |
Notes |
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WB
1/5000. For unpurified use at 1/1000 - 1/10000. |
ELISA
Use a concentration of 1 µg/ml. For unpurified use at 1/1000 - 1/10000. |
Images
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All lanes : Rabbit monoclonal [H19-5] Anti-Human kappa light chain (ab125919) at 1/5000 dilution
Lane 1 : Human spleen tissue lysate
Lane 2 : Human thymus tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Observed band size: 25 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST.
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Rabbit monoclonal [H19-5] Anti-Human kappa light chain (ab125919) at 1/5000 dilution (purified) + Human tonsil tissue lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Observed band size: 25 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST.
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Direct ELISA antibody dose-response curve using ab125919 between 0-2500 ng/ml. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.
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Direct ELISA antibody dose-response curve using ab125919 between 0-2500 ng/ml. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.
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All lanes : Rabbit monoclonal [H19-5] Anti-Human kappa light chain (ab125919) (unpurified)
Lane 1 : Human spleen tissue lysate
Lane 2 : Human thymus tissue lysate
Lane 3 : Human tonsil tissue lysate -
ELISA dose-responsive curve using purified ab125919 at a range of concentrations.
Antigen concentation: 1000ng/ml.
Secondary antibody: Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L), 1/2500.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab125919 has been referenced in 3 publications.
- Hunt AC et al. A rapid cell-free expression and screening platform for antibody discovery. Nat Commun 14:3897 (2023). PubMed: 37400446
- Zhang H et al. microRNA-4701-5p protects against interleukin-1β induced human chondrocyte CHON-001 cells injury via modulating HMGA1. J Orthop Surg Res 17:246 (2022). PubMed: 35459188
- Seo Y et al. Assembly and Folding Properties of Cytosolic IgG Intrabodies. Sci Rep 10:2140 (2020). PubMed: 32034177