Rabbit monoclonal H19-5 Anti-Human kappa light chain (ab125919)

Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 99% PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone numberH19-5
  • IsotypeIgG
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Alternative versions available:

    Anti-Human kappa light chain (Biotin) [H19-5] (ab201484)

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab125919 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000.

For unpurified use at 1/1000 - 1/10000.

ELISA Use a concentration of 1 µg/ml.

For unpurified use at 1/1000 - 1/10000.

Rabbit monoclonal H19-5 Anti-Human kappa light chain images

  • All lanes : Rabbit monoclonal H19-5 Anti-Human kappa light chain (ab125919) at 1/5000 dilution (purified)

    Lane 1 : Human spleen tissue lysate
    Lane 2 : Human thymus tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Observed band size : 25 kDa (why is the actual band size different from the predicted?)

    Blocking and diluting buffer: 5% NFDM/TBST.

  • Rabbit monoclonal H19-5 Anti-Human kappa light chain (ab125919) at 1/5000 dilution (purified) + Human tonsil tissue lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Observed band size : 25 kDa (why is the actual band size different from the predicted?)

    Blocking and diluting buffer: 5% NFDM/TBST.

  • All lanes : Rabbit monoclonal H19-5 Anti-Human kappa light chain (ab125919) (unpurified)

    Lane 1 : Human spleen tissue lysate
    Lane 2 : Human thymus tissue lysate
    Lane 3 : Human tonsil tissue lysate
  • ELISA dose-responsive curve using purified ab125919 at a range of concentrations.

    Antigen concentation: 1000ng/ml.

    Secondary antibody: Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L), 1/2500.

References for Rabbit monoclonal H19-5 Anti-Human kappa light chain (ab125919)

ab125919 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"