Recombinant
RabMAb

Rabbit monoclonal R18-2 Anti-Rat IgG Fc (ab125900)

Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 99% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    R18-2
  • Isotype
    IgG1
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab125900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.0156 - 1 µg/ml.

Images

  • Direct ELISA antibody dose-response curve using ab125900 between 0-1000 ng/ml. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

  • Antibody dose-response curve using purified ab125900. Antigen concentration of 1 µg/mL. An Alkaline Phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

References

This product has been referenced in:
  • Wittenborn TR  et al. Simulation of heterogeneous molecular delivery in tumours using µCT reconstructions and MRI validation. Microvasc Res 108:69-74 (2016). Read more (PubMed: 27569845) »
  • Fons P  et al. Tumor vasculature is regulated by FGF/FGFR signaling-mediated angiogenesis and bone marrow-derived cell recruitment: this mechanism is inhibited by SSR128129E, the first allosteric antagonist of FGFRs. J Cell Physiol 230:43-51 (2015). Read more (PubMed: 24760775) »

See all 3 Publications for this product

Customer reviews and Q&As

Thank you very much for your call last week and for your patience while I've been in touch with the lab regarding your enquiry.
The immunogen was whole rat IgG, but different subclones were generated and then were tested for specificity against F(a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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