Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97043)
- Datasheet
- Specific References (1)
- Protocols
Overview
- Product nameRabbit Anti-Mouse IgG H&L (Alkaline Phosphatase)
See all IgG secondary antibodies - Target speciesMouse
- SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
- Tested applicationsSuitable for: ICC, ELISA, IHC-P, WBmore details
- ConjugationAlkaline Phosphatase
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Store at +4°C.
- Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 7.1 -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).
- Conjugation notesMolar enzyme: antibody protein ratio is 1:1
- ClonalityPolyclonal
- IsotypeIgG
- General notesPart of the AbExcel range.
- Research areas
Applications
Our Abpromise guarantee covers the use of ab97043 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC | Use at an assay dependent dilution. | |
| ELISA | 1/5000 - 1/50000. (Primary). | |
| IHC-P | 1/100 - 1/1000. | |
| WB | 1/5000 - 1/50000. (Chemiluminescent). |
Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase) images
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Western blot - Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (AP) (ab97043)All lanes : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 1 µg/ml
Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.
Secondary
Lanes 1 - 2 : Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (AP) (ab97043) at 1/5000 dilution
Lanes 3 - 4 : Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (AP) (ab97043) at 1/20000 dilution
Lanes 5 - 6 : Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (AP) (ab97043) at 1/50000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Exposure time : 20 minutes
References for Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97043)
This product has been referenced in:
- Bergeron KF et al. Male-biased aganglionic megacolon in the TashT mouse line due to perturbation of silencer elements in a large gene desert of chromosome 10. PLoS Genet 11:e1005093 (2015). IHC ; Mouse . Read more (PubMed: 25786024) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"