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Product name Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase) See all IgG secondary antibodies
Target species Mouse
Specificity By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
Tested applications ICC, ELISA, IHC-P, WB more details
Conjugation Alkaline Phosphatase Properties
Storage instructions Shipped at 4°C. Store at +4°C.
Storage buffer Preservative: 0.09% Sodium Azide
Constituents: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 7.1
Concentration information loading...
Purity Immunogen affinity purified
Purification notes This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).
Conjugation notes Molar enzyme: antibody protein ratio is 1:1
General notes Part of the AbExcel range.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution.
1/5000 - 1/50000. (Primary).
1/100 - 1/1000.
1/5000 - 1/50000. (Chemiluminescent).
Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase) images
Western blot - Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (AP) (ab97043)
All lanes :
Anti-beta Actin antibody [mAbcam 8226] - Loading Control (
) at 1 µg/ml
Lanes 1-6 :
HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary Lanes 1 - 2 : Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (AP) (ab97043) at 1/5000 dilution Lanes 3 - 4 : Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (AP) (ab97043) at 1/20000 dilution Lanes 5 - 6 : Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (AP) (ab97043) at 1/50000 dilution Developed using the ECL technique Performed under reducing conditions. Exposure time : 20 minutes
References for Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97043)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"