Rabbit Anti-Mouse IgG H&L (Biotin) preadsorbed (ab98784)

Overview

  • Product name
    Rabbit Anti-Mouse IgG H&L (Biotin) preadsorbed
    See all IgG secondary antibodies
  • Description
    Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (Biotin), pre-adsorbed
  • Target species
    Mouse
  • Tested applications
    Suitable for: IHC-P, ICC/IF, ELISA, WBmore details
  • Minimal
    cross-reactivity

    Human, Rat more details
  • Conjugation
    Biotin

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab98784 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/5000.
ICC/IF 1/200 - 1/1000.
ELISA 1/20000 - 1/400000. as primary
WB 1/20000 - 1/400000. chemiluminescent detection

Images

  • Immunohistochemical analysis of dog skin tissue, detecting staining of NQO1 with ab98784.

    Tissue was fixed with formaldehyde and blocked with none for ; antigen retrieval was by heat mediation in a citrate buffer (pH 6). Samples were incubated with primary antibody (ab28947 Mouse monoclonal [A180] to NQO1 at 1/175 in BSA in TBS) for 45 minutes. ab98784 (1/500) was used as the secondary antibody.

    See Abreview

References

ab98784 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Since the no primary control is showing staining, it's probably an issue with the secondary antib...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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