Rabbit Anti-Mouse IgG H&L (DyLight® 550) preadsorbed (ab98786)

Overview

  • Product nameRabbit Anti-Mouse IgG H&L (DyLight® 550) preadsorbed
    See all IgG secondary antibodies
  • Target speciesMouse
  • Tested applicationsSuitable for: IHC-P, ICC/IF, Flow Cyt, WBmore details
  • Minimal
    cross-reactivity

    Human, Rat more details
  • ConjugationDyLight® 550. Ex: 562nm, Em: 576nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.
  • ClonalityPolyclonal
  • IsotypeIgG
  • General notesDyLight® 550 replaces DyLight® 549.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab98786 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500. DyLight®550 replaces DyLight®549. The DyLight®550 excitation is 562nm and the emission is 576nm. DyLight®550 provides the same orange-to-red fluorescence and photostability as the DyLight®549 dye. Please refer to the vial in order to check whether the product you received is DyLight®549 or DyLight®550. This information however will have no impact on your experiments. For more information please refer to www.abcam.com/DyLight550.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.
WB Use at an assay dependent concentration. See below Abreview

Rabbit Anti-Mouse IgG H&L (DyLight® 550) preadsorbed images

  • All lanes : Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484) at 1/1000 dilution

    Lane 1 : Rat Hippocampus
    Lane 2 : Rat Hippocampus
    Lane 3 : Rat Hippocampus

    Lysates/proteins at 25 µg per lane.

    Secondary
    Lane 1 : Rabbit Anti-Mouse IgG H&L (DyLight® 550) preadsorbed (ab98786) at 1/5000 dilution
    Lane 2 : Rabbit Anti-Mouse IgG H&L (DyLight® 550) preadsorbed (ab98786) at 1/1000 dilution
    Lane 3 : Other Commercial Dye at 1/1000 dilution


    Performed under reducing conditions.

    This image is courtesy of an anonymous abreview.

    See Abreview

  • Anti-HuC/D staining in mouse Formalin Fixed Paraffin Embedded sections. For antigen retrieval HIER in citrate buffer was performed. Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 550), pre-adsorbed (ab98786) was used as a secondary antibody at a concentration of 1/500.
  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References for Rabbit Anti-Mouse IgG H&L (DyLight® 550) preadsorbed (ab98786)

ab98786 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Immunocytochemistry/ Immunofluorescence
Red channel is anti-mouse Dy550, label pre-synaptic marker Bassoon. work at dilution of 1:500, incubate in 2%BSA/PBS O/N in Fridge.
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Submitted Feb 28 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"