Overview

  • Product nameRabbit Anti-Mouse IgG H&L (FITC) preadsorbed
    See all IgG secondary antibodies
  • Target speciesMouse
  • Tested applicationsSuitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISAmore details
  • Minimal
    cross-reactivity

    Human more details
  • Immunogen

    Full length native Mouse IgG (purified).

  • ConjugationFITC. Ex: 493nm, Em: 528nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notesDiaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 3.3 moles DTAF per mole of Rabbit IgG
  • ClonalityPolyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Immunomicroscopy
Flow Cyt
IHC-P
IHC-Fr
ELISA
  • Application notesFlow Cyt: Use at an assay dependant concentration.
    IHC-P: Use at an assay dependant concentration.
    IHC-Fr: Use at an assay dependant concentration.
    IM: Use at an assay dependant concentration.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References for Rabbit Anti-Mouse IgG H&L (FITC) preadsorbed (ab7071)

    This product has been referenced in:
    • Bock F  et al. Activated protein C ameliorates diabetic nephropathy by epigenetically inhibiting the redox enzyme p66Shc. Proc Natl Acad Sci U S A 110:648-53 (2013). Read more (PubMed: 23267072) »
    • Blakeslee D & Baines MG Immunofluorescence using dichlorotriazinylaminofluorescein (DTAF). I. Preparation and fractionation of labelled IgG. J Immunol Methods 13:305-20 (1976). Read more (PubMed: 13125) »

    See all 2 Publications for this product

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    There are currently no Abreviews or Questions for ab7071.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"