Overview

  • Product name
    Rabbit Anti-Mouse IgG H&L (HRP)
    See all IgG secondary antibodies
  • Description
    Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP)
  • Target species
    Mouse
  • Tested applications
    Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, ICC/IF, WBmore details
  • Immunogen

    Mouse IgG whole molecule

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads.
  • Conjugation notes
    Horseradish Peroxidase (HRP)
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes
    Many of our customers have reported seeing brown precipitates in the vials. The brown precipitates are very common with HRP conjugated antibodies; we suggest vortexing the vial and using this antibody as normal. Our customer’s feedback says the antibody worked great. If in case the antibody fails to give results then please contact our Scientific Support team for assistance.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6728 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent dilution.
ELISA 1/1000.
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent dilution.
ICC/IF 1/1000 - 1/5000.
WB 1/2000 - 1/10000. PubMed: 17200442

Rabbit Anti-Mouse IgG H&L (HRP) images

  • ab6728 was used at dilution 1/2000 with the primary antibody ab72009 in WB. See the review on ab72009.

  • Ab6728 was used at dilution 1/200 with the primary antibody ab81785 in IHC-P. See the review on ab81785.
  • Ab6728 was used at dilution 1/500 with the primary antibody ab3089 in IHC-Fr (PFA perfusion). See the review on ab3089.
  • Ab6728 was used at dilution 1/1000 with the primary antibody ab11038 in ELISA. See the review on ab11038.

References for Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)

This product has been referenced in:
  • Zhang P  et al. miR-600 inhibits cell proliferation, migration and invasion by targeting p53 in mutant p53-expressing human colorectal cancer cell lines. Oncol Lett 13:1789-1796 (2017). WB . Read more (PubMed: 28454325) »
  • Yang X  et al. MicroRNA-133b inhibits the migration and invasion of non small cell lung cancer cells via targeting FSCN1. Oncol Lett 12:3619-3625 (2016). WB . Read more (PubMed: 27900045) »

See all 68 Publications for this product

Product Wall

Abreviews
Application
Western blot
A excellent 2nd Ab for WB (1:2,000 dilution) used with ab9484 (1:10000)
Username

Mr. Baptiste Jude

Verified customer

Submitted Oct 10 2016

Abreviews
Application
Western blot
I used this antibody, when I perform WB.
This antibody is very sensitive.
Dilution concentration that I used is 1/2000 in TBST(5% BSA) for 1hour.
Username

Abcam user community

Verified customer

Submitted May 30 2014

Abreviews
Application
Western blot
When I perform WB, that is very sensitive. making band is clearly
Username

Abcam user community

Verified customer

Submitted Jan 23 2014

Abreviews
Application
Western blot
strong detection
Username

Abcam user community

Verified customer

Submitted Jul 11 2013

Thank you for your email. I am sorry to hear that customer have been experiencing problems with this antibody.

I have read the details you have kindly provided and have following further suggestions for troubleshooting;

- Please try 5...

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Thank you for contacting us. I am sorry to hear these antibodies are not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I apprec...

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Thank you for your message.

I am pleased to help answer your question.

I can confirm that '1 mg at 2mg/ml' means that the vial contains 1 mg of antibody at a concentration of 2 mg.ml. The concentration is 2 mg/ml. (Therefore the v...

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Thank you for your reply.

I can confirm that for the HRP conjugated antibodies the approach that Irecommended is the correct one. Spining the vial down will prevent free HRP to bind to un-specifically to samples and therefore avoid increased ...

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Thank you for your enquiry. I am sorry to hear the customer has concerns regarding the quality of ab6721, ab6741, ab6789 and ab6728.

As the antibody does contain proteins and other solutes it would not be abnormal to see a little cloudiness ...

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Thank you for contacting Abcam.

I feel that both products are suitable for IHC-P. Both have many specific references and in-depth scientific support data right on the datasheet. They differ mainly in the species in which they were raised and i...

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1-10 of 35 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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