Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) (ab8517)
- Product nameRabbit Anti-Mouse IgG+IgM+IgA H&L (FITC)See all IgG + IgM + IgA secondary antibodies ...
- DescriptionRabbit polyclonal Secondary Antibody to Mouse IgG+IgM+IgA (FITC)
- Target speciesMouse
- SpecificityAntibodies were raised in rabbit to mouse IgG, IgM and IgA
- Tested applicationsICC/IF, IHC-P more details
- ConjugationFITC. Ex: 493nm, Em: 528nm
- Storage instructionsStore at +4°C.
- Storage buffer50% glycerol, 0.1 M NaCl, 0.01 M sodium phosphate at pH 7.5.
- Concentration information loading...
- PurityImmunogen affinity purified
- Purification notesSpecific antibodies were absorpted by incubation with Sepharose-bound mouse immunoglobulins. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized mouse immunoglobulins a minimum of 65% protein bound.
- Conjugation notesThe purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate.
- Clonality Polyclonal
- General notesF/P ratio is 3-5 mol Fluorescein per 1 mol of rabbit Ig. There is low background fluorescence of normal tissue in contrast to specific staining of sections treated with monoclonal mouse anti-human collagen antibodies
- Research Areas
Our Abpromise guarantee covers the use of ab8517 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||ICC/IF: Use at an assay dependent dilution. PubMed: 15483223|
|IHC-P||IHC-P: Use at an assay dependent dilution.|
Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) images
References for Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) (ab8517)
This product has been referenced in:
- Guo Z et al. Antisense oligonucleotide treatment enhances the recovery of acute lung injury through IL-10-secreting M2-like macrophage-induced expansion of CD4+ regulatory T cells. J Immunol 190:4337-48 (2013). Read more (PubMed: 23514739) »
- Tang L et al. Characterization of mesenchymal stem cells from human normal and hyperplastic gingiva. J Cell Physiol 226:832-42 (2011). IHC-P . Read more (PubMed: 20857425) »