Storage instructionsShipped at 4°C. Store at +4°C.
Storage buffer50% glycerol, 0.1 M NaCl, 0.01 M sodium phosphate at pH 7.5.
Concentration information loading...
PurityImmunogen affinity purified
Purification notesSpecific antibodies were absorpted by incubation with Sepharose-bound mouse immunoglobulins.
Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis.
After repeated binding with immobilized mouse immunoglobulins a minimum of 65% protein bound.
Conjugation notesThe purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate.
General notesF/P ratio is 3-5 mol Fluorescein per 1 mol of rabbit Ig. There is low background fluorescence of normal tissue in contrast to specific staining of sections treated with monoclonal mouse anti-human collagen antibodies
Ab8517 was used at dilution 1/200 with the primary antibody ab18230 in ICC/IF. See the review on ab18230.
References for Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) (ab8517)
This product has been referenced in:
Guo Z et al. Antisense oligonucleotide treatment enhances the recovery of acute lung injury through IL-10-secreting M2-like macrophage-induced expansion of CD4+ regulatory T cells. J Immunol190:4337-48 (2013).
Read more (PubMed: 23514739) »
Tang L et al. Characterization of mesenchymal stem cells from human normal and hyperplastic gingiva. J Cell Physiol226:832-42 (2011).
Read more (PubMed: 20857425) »