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Products:Secondary antibodies >> anti-Chicken >> IgY >> Biotin
Overview
Product name
Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (Biotin)
See all IgY products (89) ...
Target species
Chicken
Tested applications
IHC-Fr, Immunomicroscopy, WB, Dot Blot, ELISA, IHC-P, ICC/IFmore details
Immunogen
chicken IgY whole molecule
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule.
Conjugation notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
Clonality
Polyclonal
Research areas
Secondary antibodies >> anti-Chicken >> IgY >> Biotin
Applications
Show applications key
Our Abpromise guarantee covers the use of ab6752 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at an assay dependent concentration.
IM: Use at an assay dependent concentration.
WB
WB: 1/2000 - 1/20000.WB: 1/2000 - 1/20000.
Dot: Use at an assay dependent concentration.
ELISA
ELISA: 1/500000ELISA: 1/500000
IHC-P: Use at an assay dependent concentration.
ICC/IF: 1/1000 - 1/5000.
Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (Biotin) images:
Immunocytochemistry/ Immunofluorescence - Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (Biotin) (ab6752)
Ab6752 was used with the primary antibody ab13989 in ICC/IF. See Abreview on ab13989.
Immunohistochemistry (Frozen sections) - Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (Biotin) (ab6752)
Ab6752 was used at dilution 1/1000 with the primary antibody ab13970 in IHC-Fr. See the review on ab13970.
References for Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (Biotin) (ab6752)
This product has been referenced in:
- Gakis Get al. XPA-210: a new proliferation marker to characterize tumor biology and progression of renal cell carcinoma. World J Urol 29:801-6 (2011). IHC-P; Human.Read more (PubMed: 21113600) »
- Wong EWet al. Regulation of blood-testis barrier dynamics by TGF-beta3 is a Cdc42-dependent protein trafficking event. Proc Natl Acad Sci U S A 107:11399-404 (2010). IHC-Fr.Read more (PubMed: 20534521) »
See all 4 publications for this product
Publishing research using ab6752? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"