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Products:Immunology >> Immunoglobulins >> Heavy Chain >> IgG
Overview
Product name
Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP)
See all IgG products (1691) ...
Target species
Mouse
Tested applications
Dot Blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, ICC/IF, WBmore details
Immunogen
Mouse IgG whole molecule
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50%, aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Gentamicin Sulfate
Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads.
Conjugation notes
Horseradish Peroxidase (HRP)
Clonality
Polyclonal
General notes
Many of our customers have reported seeing brown precipitates in the vials. The brown precipitates are very common with HRP conjugated antibodies; we suggest vortexing the vial and using this antibody as normal. Our customer’s feedback says the antibody worked great. If in case the antibody fails to give results then please contact our Scientific Support team for assistance.
Research areas
Secondary antibodies >> anti-Mouse >> IgG >> Enzyme >> HRP
Immunology >> Immunoglobulins >> Heavy Chain >> IgG
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- Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6728)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6728)
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6728)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab6728 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Dot: Use at an assay dependent dilution.
ELISA: 1/200000
IHC-P: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
IM: Use at an assay dependent dilution.
ICC/IF
ICC/IF: 1/1000 - 1/5000.ICC/IF: 1/1000 - 1/5000.
WB: 1/2000 - 1/10000.(PubMed: 17200442)
Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) images:
- Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6728)
Ab6728 was used at dilution 1/2000 with the primary antibody ab72009 in WB. See the review on ab72009.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6728)
Ab6728 was used at dilution 1/200 with the primary antibody ab81785 in IHC-P. See the review on ab81785.
Immunohistochemistry (PFA perfusion fixed frozen sections) - Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6728)
Ab6728 was used at dilution 1/500 with the primary antibody ab3089 in IHC-Fr (PFA perfusion). See the review on ab3089.
ELISA - Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6728)
Ab6728 was used at dilution 1/1000 with the primary antibody ab11038 in ELISA. See the review on ab11038.
References for Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6728)
This product has been referenced in:
- Benneyworth MAet al. Failure of NMDA receptor hypofunction to induce a pathological reduction in PV-positive GABAergic cell markers. Neurosci Lett 488:267-71 (2011). WB.Read more (PubMed: 21094213) »
- Hess Met al. Bacterial glucuronidase as general marker for oncolytic virotherapy or other biological therapies. J Transl Med 9:172 (2011). WB.Read more (PubMed: 21989091) »
See all 18 publications for this product
Publishing research using ab6728? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"