Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Immunology >> Immunoglobulins >> Heavy Chain >> IgG
Overview
Product name
Rabbit anti-Mouse IgG + IgM + IgA (FITC) secondary antibody
See all IgG + IgM + IgA products (59) ...
Description
Rabbit polyclonal Secondary Antibody to Mouse IgG+IgM+IgA (FITC)
Target species
Mouse
Specificity
Antibodies were raised in rabbit to mouse IgG, IgM and IgA
Tested applications
ICC/IF, IHC-P, IFmore details
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
50% glycerol, 0.1 M NaCl, 0.01 M sodium phosphate at pH 7.5.
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Specific antibodies were absorpted by incubation with Sepharose-bound mouse immunoglobulins. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized mouse immunoglobulins a minimum of 65% protein bound.
Conjugation notes
The purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate.
Clonality
Polyclonal
General notes
F/P ratio is 3-5 mol Fluorescein per 1 mol of rabbit Ig. There is low background fluorescence of normal tissue in contrast to specific staining of sections treated with monoclonal mouse anti-human collagen antibodies
Research areas
Secondary antibodies >> anti-Mouse >> IgG + IgM + IgA >> Fluorophore >> FITC
Immunology >> Immunoglobulins >> Heavy Chain >> IgM
Immunology >> Immunoglobulins >> Heavy Chain >> IgA
Immunology >> Immunoglobulins >> Heavy Chain >> IgG
-
Immunocytochemistry - Rabbit polyclonal Secondary Antibody to Mouse IgG+IgM+IgA (FITC) (ab8517)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab8517 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution. (PubMed: 15483223)
IHC-P
IHC-P: 1/40IHC-P: 1/40
IF: Use at an assay dependent concentration.
Rabbit anti-Mouse IgG + IgM + IgA (FITC) secondary antibody images:
Immunocytochemistry - Rabbit polyclonal Secondary Antibody to Mouse IgG+IgM+IgA (FITC) (ab8517)
Ab8517 was used at dilution 1/200 with the primary antibody ab18230 in ICC/IF. See the review on ab18230.
References for Rabbit anti-Mouse IgG + IgM + IgA (FITC) secondary antibody (ab8517)
This product has been referenced in:
- Tang Let al. Characterization of mesenchymal stem cells from human normal and hyperplastic gingiva. J Cell Physiol 226:832-42 (2011). IHC-P.Read more (PubMed: 20857425) »
- Huang CYet al. Plasticity of stem cells derived from adult periodontal ligament. Regen Med 4:809-21 (2009).Read more (PubMed: 19903001) »
See all 4 publications for this product
Publishing research using ab8517? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"