Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Immunology >> Immunoglobulins >> Heavy Chain >> IgG
Overview
Product name
Rabbit polyclonal Secondary Antibody to Sheep IgG - H&L (Biotin)
See all IgG products (1691) ...
Target species
Sheep
Tested applications
WB, ELISA, Immunomicroscopy, Dot Blot, ICC/IF, IHC-P, IHC-Frmore details
Immunogen
Sheep IgG whole molecule
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule
Conjugation notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
Clonality
Polyclonal
Research areas
Secondary antibodies >> anti-Sheep >> IgG >> Biotin
Immunology >> Immunoglobulins >> Heavy Chain >> IgG
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit polyclonal Secondary Antibody to Sheep IgG - H&L (Biotin) (ab6746)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab6746 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
WB: 1/1000 - 1/5000.WB: 1/1000 - 1/5000.
ELISA
ELISA: 1/140000ELISA: 1/140000
IM: Use at an assay dependent concentration.
Dot: Use at an assay dependent concentration.
ICC/IF
ICC/IF: 1/500 - 1/3000.ICC/IF: 1/500 - 1/3000.
IHC-P: Use at an assay dependent concentration.
IHC-Fr: Use at an assay dependent concentration.
Rabbit polyclonal Secondary Antibody to Sheep IgG - H&L (Biotin) images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit polyclonal Secondary Antibody to Sheep IgG - H&L (Biotin) (ab6746)
Ab6746 was used at dilution 1/500 with the primary antibody ab9013 in IHC-P. See the review on ab9013.
References for Rabbit polyclonal Secondary Antibody to Sheep IgG - H&L (Biotin) (ab6746)
This product has been referenced in:
- Lindersson Eet al. p25alpha Stimulates alpha-synuclein aggregation and is co-localized with aggregated alpha-synuclein in alpha-synucleinopathies. J Biol Chem 280:5703-15 (2005).Read more (PubMed: 15590652) »
See 1 publication for this product
Publishing research using ab6746? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"