Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (ab102169)
Key features and details
- Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase)
- Conjugation: Alkaline Phosphatase
- Host species: Rabbit
- Isotype: IgG
- Suitable for: IHC-P, ICC, ELISA, WB
Overview
-
Product name
Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase)
See all IgG secondary antibodies -
Host species
Rabbit -
Target species
Rat -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species. -
Tested applications
Suitable for: IHC-P, ICC, ELISA, WBmore details -
Conjugation
Alkaline Phosphatase
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.58% Sodium chloride, 0.0095% Magnesium chloride, 0.00136% Zinc chloride, 1.19% HEPES, 0.2% BSA -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab102169 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/100 - 1/1000.
|
|
ICC |
1/100 - 1/1000.
|
|
ELISA |
1/1000 - 1/10000. (Primary ELISA).
|
|
WB |
1/1000 - 1/10000. (Chemiluminescent).
|
Notes |
---|
IHC-P
1/100 - 1/1000. |
ICC
1/100 - 1/1000. |
ELISA
1/1000 - 1/10000. (Primary ELISA). |
WB
1/1000 - 1/10000. (Chemiluminescent). |
Images
-
All lanes : Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) at 1 µg/ml (Anti-Tubulin antibody [YL1/2] - Loading Control)
All lanes : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1-2 : Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (ab102169) at 1/2000 dilution (Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP))
Lanes 3-4 : Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (ab102169) at 1/10000 dilution (Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP))
Lanes 5-6 : Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (ab102169) at 1/20000 dilution (Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP))
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 52 kDa why is the actual band size different from the predicted?
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (0)
ab102169 has not yet been referenced specifically in any publications.