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Product name Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) See all IgG secondary antibodies
Target species Rat
Specificity By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
Tested applications Suitable for:
IHC-P, ICC, ELISA, WB more details
Conjugation Alkaline Phosphatase Properties
Storage instructions Shipped at 4°C. Store at +4°C.
Storage buffer Preservative: 0.09% Sodium Azide
Constituents: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 7.1
Concentration information loading...
Purity Immunogen affinity purified
Purification notes This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/1000.
1/100 - 1/1000.
1/1000 - 1/10000. (Primary ELISA).
1/1000 - 1/10000. (Chemiluminescent).
Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) images
Western blot - Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (AP) (ab102169)
All lanes : Anti-Tubulin antibody [YL1/2] - Loading Control ( ab6160) at 1 µg/ml Lanes 1-6: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary Lanes 1 - 2 : Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab102169) at 1/2000 dilution Lanes 3 - 4 : Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab102169) at 1/10000 dilution Lanes 5 - 6 : Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab102169) at 1/20000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size : 50 kDa Observed band size : 52 kDa
Additional bands at : 85 kDa. We are unsure as to the identity of these extra bands. Exposure time : 3 minutes
Abcam recommends blocking with 3% milk to reproduce this western blot data.
References for Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (ab102169)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"