Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (ab102169)

Overview

  • Product nameRabbit Anti-Rat IgG H&L (Alkaline Phosphatase)
    See all IgG secondary antibodies
  • Target speciesRat
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
  • Tested applicationsIHC-P, ICC, ELISA, WBmore details
  • ConjugationAlkaline Phosphatase

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 7.1
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab102169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
ICC 1/100 - 1/1000.
ELISA 1/1000 - 1/10000. (Primary ELISA).
WB 1/1000 - 1/10000. (Chemiluminescent).

Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) images

  • All lanes : Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) at 1 µg/ml

    Lanes 1-6:  HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lanes 1 - 2 : Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab102169) at 1/2000 dilution
    Lanes 3 - 4 : Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab102169) at 1/10000 dilution
    Lanes 5 - 6 : Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab102169) at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 52 kDa

    Additional bands at : 85 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes

    Abcam recommends blocking with 3% milk to reproduce this western blot data. 

References for Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (ab102169)

ab102169 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"