Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6735)

Overview

  • Product nameRabbit Anti-Rat IgG H&L (Alkaline Phosphatase)
    See all IgG secondary antibodies
  • Target speciesRat
  • Tested applicationsSuitable for: IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-Frmore details
  • Immunogen

    Rat IgG whole molecule

  • ConjugationAlkaline Phosphatase

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 8
    Preservative: 0.01% Sodium azide
    Constituents: 0.87% Sodium chloride, 0.788% Tris HCl, 0.0095% Magnesium chloride, 0.014% Zinc chloride, 50% Glycerol, 1% BSA
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads.
  • Conjugation notesAlkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
  • ClonalityPolyclonal
  • General notesPart of the AbExcel range.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6735 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/5000 - 1/50000.
ELISA Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
ICC/IF 1/200 - 1/1000.
IHC-Fr Use at an assay dependent concentration.

Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) images

  • All lanes : Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) at 1 µg/ml

    Lanes 1-6:  HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lanes 1 - 2 : Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab6735) at 1/2000 dilution
    Lanes 3 - 4 : Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab6735) at 1/10000 dilution
    Lanes 5 - 6 : Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab6735) at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 52 kDa

    Additional bands at : 85 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

    Abcam recommends blocking with 3% milk to reproduce this western blot data. 

References for Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6735)

ab6735 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"