• Product nameRabbit Anti-Rat IgG H&L (Biotin)
    See all IgG secondary antibodies
  • Description
    Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (Biotin)
  • Target speciesRat
  • Tested applicationsSuitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Frmore details
  • Immunogen

    Rat IgG whole molecule

  • ConjugationBiotin


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notesBiotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab6733 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.
ELISA 1/500000.
Immunomicroscopy Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
ICC/IF 1/1000 - 1/5000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Rabbit Anti-Rat IgG H&L (Biotin) images

  • Ab6640 (Rat monoclonal [CI:A3-1] to F4/80) at a 1/8000 dilution staining mouse spleen tissue by IHC-P. Ab6733 was used as the secondary antibody at a dilution of 1/400.

    See Abreview

  • Ab6733 was used at dilution 1/100 with the primary antibody ab25375 in IHC-Fr. See the review on ab25375.

References for Rabbit Anti-Rat IgG H&L (Biotin) (ab6733)

This product has been referenced in:
  • Lucas AR  et al. Myxomavirus anti-inflammatory chemokine binding protein reduces the increased plaque growth induced by chronic Porphyromonas gingivalis oral infection after balloon angioplasty aortic injury in mice. PLoS One 9:e111353 (2014). IHC ; Mouse . Read more (PubMed: 25354050) »
  • Lee HJ  et al. Oral administration of penta-O-galloyl-{beta}-D-glucose suppresses triple-negative breast cancer xenograft growth and metastasis in strong association with JAK1-STAT3 inhibition. Carcinogenesis 32:804-11 (2011). IHC . Read more (PubMed: 21289371) »

See all 6 Publications for this product

Product Wall

Merci de nous avoir contactés.

Nous sommes désolés d'apprendre que le produit ab6733 que vous avez reçu ne fonctionne pas comme attendu.
Comme convenu j'ai mis en place l'envoi d'une unité de ab7103 (http...

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Thank you for your interest in Abcam products. ab2378, ab955 and ab9018 are mouse monoclonal antibodies. I would recommend using ab97044 as a secondary antibody; http://www.abcam.com/Rabbit-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-Biotin-ab970...

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I recommend ab7096 -- Goat polyclonal to rat IgG H&L (biotin) Pre-Adsorbed