Overview

  • Product name
    Rabbit Anti-Rat IgG H&L (FITC)
    See all IgG secondary antibodies
  • Target species
    Rat
  • Tested applications
    Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details
  • Immunogen

    Rat whole molecule

  • Conjugation
    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunomicroscopy Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.
ICC/IF 1/1000 - 1/5000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ELISA 1/10000 - 1/50000.

Rabbit Anti-Rat IgG H&L (FITC) images

  • Ab6730 was used at dilution 1/500 with the primary antibody ab53444 in IHC-Fr. See the review on ab53444.

References for Rabbit Anti-Rat IgG H&L (FITC) (ab6730)

This product has been referenced in:
  • Sun KH  et al. Evaluation of in vitro and in vivo biocompatibility of a myo-inositol hexakisphosphate gelated polyaniline hydrogel in a rat model. Sci Rep 6:23931 (2016). ICC/IF . Read more (PubMed: 27073144) »
  • Zhong SY  et al. Low-dose levodopa protects nerve cells from oxidative stress and up-regulates expression of pCREB and CD39. PLoS One 9:e95387 (2014). ICC/IF, WB . Read more (PubMed: 24743653) »

See all 5 Publications for this product

Product Wall

Secondary ab

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
It works good with abcam as well as other vendors antibodies
Username

Abcam user community

Verified customer

Submitted Sep 05 2014

Thank you for your recent enquiry. For immunohistochemistry, I would recommend simply omitting the primary antibody step as a control experiment. Indeed any of the secondary antibodies recommended on the datasheet of ab15684 will work (in my view C...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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