Overview

  • Product name
    Rabbit Anti-Rat IgG H&L (FITC)
    See all IgG secondary antibodies
  • Host species
    Rabbit
  • Target species
    Rat
  • Tested applications
    Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details
  • Immunogen

    Rat whole molecule

  • Conjugation
    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunomicroscopy Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.
ICC/IF 1/1000 - 1/5000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ELISA 1/10000 - 1/50000.

Images

  • Ab6730 was used at dilution 1/500 with the primary antibody ab53444 in IHC-Fr. See the review on ab53444.

References

This product has been referenced in:
  • Kneppers A  et al. A novel in vitro model for the assessment of postnatal myonuclear accretion. Skelet Muscle 8:4 (2018). Read more (PubMed: 29444710) »
  • Gholinejad M  et al. Adenosine decreases oxidative stress and protects H2O2-treated neural stem cells against apoptosis through decreasing Mst1 expression. Biomed Rep 8:439-446 (2018). Read more (PubMed: 29732147) »

See all 7 Publications for this product

Customer reviews and Q&As

Secondary ab

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
It works good with abcam as well as other vendors antibodies
Username

Abcam user community

Verified customer

Submitted Sep 05 2014

Thank you for your recent enquiry. For immunohistochemistry, I would recommend simply omitting the primary antibody step as a control experiment. Indeed any of the secondary antibodies recommended on the datasheet of ab15684 will work (in my view C...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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