Rabbit Anti-Rat IgG H&L (FITC) preadsorbed (ab7100)

Overview

  • Product name
    Rabbit Anti-Rat IgG H&L (FITC) preadsorbed
    See all IgG secondary antibodies
  • Target species
    Rat
  • Tested applications
    Suitable for: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cyt, ELISAmore details
  • Minimal
    cross-reactivity

    Human more details
  • Immunogen

    Full length native Rat IgG (purified).

  • Conjugation
    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.40
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
IHC-P
IHC-Fr
Immunomicroscopy
Flow Cyt
ELISA
  • Application notes
    Flow Cyt: Use at an assay dependant concentration.
    IHC-P: Use at an assay dependant concentration.
    IHC-Fr: Use at an assay dependant concentration.
    IM: Use at an assay dependant concentration.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References for Rabbit Anti-Rat IgG H&L (FITC) preadsorbed (ab7100)

    ab7100 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you very much for your reply.

    I've looked at the package'stracking information, and it arrived on September 20 at 9:34 EST. You can track the package on FedEx.com with tracking number: ***. It was signed for by ***- does that help you t...

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    Thank you very much for your reply.

    It does sound like the secondary is reacting with the IgG's in the mouse serum. I'm sending a free of charge vial of ab6115 on the order *** which should arrive tomorrow.

    Please keep me updated ab...

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    Thank you very much for contacting us with your question.

    It is possible that the secondary antibody is causing some background signal, as ab7100 has been pre-adsorbed only against human serum proteins and not mouse serum proteins. Have you d...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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