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By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
Shipped at 4°C. Store at +4°C.
Preservative: 0.1% Proclin
Constituents: 0.2% BSA, PBS
Concentration information loading...
Immunogen affinity purified
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to HRP.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200 - 1/5000.
1/200 - 1/5000.
1/10000 - 1/100000. (as primary)
1/2000 - 1/20000. (Colorimetric: 1/5000 - 1/30000, Chemiluminescent: 1/10000 - 1/50000).
Western blot - Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab102172)
All lanes : Anti-Tubulin antibody [YL1/2] - Loading Control ( ab6160) at 1 µg/ml Lanes 1-6: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary Lanes 1 - 2 : Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab102172) at 1/2000 dilution Lanes 3 - 4 : Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab102172) at 1/10000 dilution Lanes 5 - 6 : Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab102172) at 1/20000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size : 50 kDa Observed band size : 52 kDa Additional bands at : 85 kDa. We are unsure as to the identity of these extra bands.
has not yet been referenced specifically in any publications.
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