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Product name Rabbit Anti-Rat IgG H&L (HRP) See all IgG secondary antibodies
Target species Rat
Specificity By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
Tested applications Suitable for:
IHC-P, ICC, ELISA, WB more details
Conjugation HRP Properties
Storage instructions Shipped at 4°C. Store at +4°C.
Storage buffer Preservative: 0.1% Proclin
Constituents: 0.2% BSA, PBS
Concentration information loading...
Purity Immunogen affinity purified
Purification notes This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to HRP.
General notes Part of the AbExcel range.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200 - 1/5000.
1/200 - 1/5000.
1/10000 - 1/100000. (as primary)
1/2000 - 1/20000. (Colorimetric: 1/5000 - 1/30000, Chemiluminescent: 1/10000 - 1/50000).
Rabbit Anti-Rat IgG H&L (HRP) images
Western blot - Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab102172)
All lanes : Anti-Tubulin antibody [YL1/2] - Loading Control ( ab6160) at 1 µg/ml Lanes 1-6: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary Lanes 1 - 2 : Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab102172) at 1/2000 dilution Lanes 3 - 4 : Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab102172) at 1/10000 dilution Lanes 5 - 6 : Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab102172) at 1/20000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size : 50 kDa Observed band size : 52 kDa Additional bands at : 85 kDa. We are unsure as to the identity of these extra bands.
References for Rabbit Anti-Rat IgG H&L (HRP) (ab102172)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"