Overview

  • Product nameRabbit Anti-Rat IgG H&L (HRP)
    See all IgG secondary antibodies
  • Description
    Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (HRP)
  • Target speciesRat
  • Tested applicationsIHC-P, WB, ELISA, Immunomicroscopy, Dot Blot, ICC/IF, IHC-Frmore details
  • Immunogen

    Rat IgG whole molecule

  • ConjugationHRP

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notesHorseradish Peroxidase (HRP)
  • Clonality Polyclonal
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab6734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/1000 - 1/5000.
ELISA 1/60000.
Immunomicroscopy Use at an assay dependent concentration.
Dot Blot Use at an assay dependent concentration.
ICC/IF 1/500 - 1/2500.
IHC-Fr Use at an assay dependent concentration.

Rabbit Anti-Rat IgG H&L (HRP) images

  • ab25377 staining Ly6g in mouse placenta tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS/1% BSA) for 12 hours at 4°C. A HRP-conjugated rabbit anti-rat IgG H&L (ab6734) (1/200) was used as the secondary antibody.

    See Abreview

  • ab6326 staining BrdU in mouse small intestine tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with protein blocking solution for 1 hour at room temperature. Samples were incubated with primary antibody (1/100) in Ab diluent for 1 hour. A HRP-conjugated rabbit anti-rat IgG H&L (ab6734) (1/500) was used as the secondary antibody.

    See Abreview

  • IHC image of alpha tubulin staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab64332, 5µg/ml overnight at +4°C. An HRP-conjugated secondary (ab6734, 1/500 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Ab6734 was used at dilution 1/5000 with the primary antibody ab33731 in WB. See the review on ab33731.

References for Rabbit Anti-Rat IgG H&L (HRP) (ab6734)

This product has been referenced in:
  • Epis MR  et al. miR-331-3p regulates expression of neuropilin-2 in glioblastoma. J Neurooncol 116:67-75 (2014). WB ; Mouse . Read more (PubMed: 24142150) »
  • Okamoto M  et al. Stem Cell Antigen 1-Positive Mesenchymal Cells Are the Origin of Follicular Cells during Thyroid Regeneration. PLoS One 8:e80801 (2013). IHC-P . Read more (PubMed: 24278321) »

See all 10 Publications for this product

Product Wall

Abreviews
Application Immunohistochemistry (PFA fixed)
worked good for DAB staining in placenta for neutrophils at 1:200 dilution for 1 hrs.
Username

Abcam user community

Verified customer

Submitted Nov 13 2013

Application Western blot
Secondary antibody worked very well. Clear bands seen. Other marks on film are pen marks only.
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Dr. Sarah Ritson

Verified customer

Submitted Oct 31 2013

Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vita...

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I would like to reassure you that these products are safe to use.

The anti-Fibrillarin antibody [38F3] is a tissue culture supernatant and is not purified. Other proteins will be within the solution and this can give it an off white colour. ...

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Thank you for your enquiry. I have received a reply from the originator of ab6734 and ab7104. For both products, there are approximately two HRPs per antibody. The method we use for our HRP conjugation is proprietary. ab6734 is only tested aga...

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Thank you for your email. Our source for this antibody tested ab11564 in Western blotting with recombinant TNF alpha. Conditions were standard. To use this antibody in Western blotting, I would recommend referring to our general Western blot protocol w...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"