Rabbit Anti-Rat IgG H&L preadsorbed (ab102248)
Key features and details
- Rabbit Anti-Rat IgG H&L preadsorbed
- Host species: Rabbit
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
Overview
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Product name
Rabbit Anti-Rat IgG H&L preadsorbed
See all IgG secondary antibodies -
Host species
Rabbit -
Target species
Rat -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, horse, human, mouse, rabbit and sheep IgG was detected. This antibody may cross react with IgG from other species. -
Tested applications
Suitable for: ICC, IHC-P, ELISA, WBmore details -
Minimal
cross-reactivity
Cow, Horse, Human, Mouse, Rabbit, Sheep more details
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antiserum was cross adsorbed using bovine, horse, human, mouse and sheep immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab102248 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
1/200 - 1/2000.
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IHC-P |
1/200 - 1/2000.
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ELISA |
1/100 - 1/30000. Coating: Use 1/100 - 1/500 dilution; Primary: Use 1/1000 - 1/30000 dilution.
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|
WB |
1/1000 - 1/30000. Colorimetric: Use 1/1000 - 1/10000 dilution; Chemiluminescent: Use 1/1000 - 1/30000 dilution.
|
Notes |
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ICC
1/200 - 1/2000. |
IHC-P
1/200 - 1/2000. |
ELISA
1/100 - 1/30000. Coating: Use 1/100 - 1/500 dilution; Primary: Use 1/1000 - 1/30000 dilution. |
WB
1/1000 - 1/30000. Colorimetric: Use 1/1000 - 1/10000 dilution; Chemiluminescent: Use 1/1000 - 1/30000 dilution. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab102248 has been referenced in 6 publications.
- Chlipala EA et al. Impact of Preanalytical Factors During Histology Processing on Section Suitability for Digital Image Analysis. Toxicol Pathol 49:755-772 (2021). PubMed: 33251977
- Dhall S et al. A Flowable Placental Formulation Prevents Bleomycin-Induced Dermal Fibrosis in Aged Mice. Int J Mol Sci 21:N/A (2020). PubMed: 32545915
- Calzavarini S et al. Platelet protein S limits venous but not arterial thrombosis propensity by controlling coagulation in the thrombus. Blood 135:1969-1982 (2020). PubMed: 32276277
- Marcher AB et al. Transcriptional regulation of Hepatic Stellate Cell activation in NASH. Sci Rep 9:2324 (2019). PubMed: 30787418
- Ziffels B et al. Antibody-Based Delivery of Cytokine Payloads to Carbonic Anhydrase IX Leads to Cancer Cures in Immunocompetent Tumor-Bearing Mice. Mol Cancer Ther 18:1544-1554 (2019). PubMed: 31213507
- Jendresen C et al. The Alzheimer's disease risk factors apolipoprotein E and TREM2 are linked in a receptor signaling pathway. J Neuroinflammation 14:59 (2017). PubMed: 28320424