Overview

  • Product name
    Rabbit Anti-Rat IgG H&L preadsorbed
    See all IgG secondary antibodies
  • Description
    Rabbit polyclonal Secondary Antibody to Rat IgG - H&L, pre-adsorbed
  • Target species
    Rat
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, horse, human, mouse, rabbit and sheep IgG was detected. This antibody may cross react with IgG from other species.
  • Tested applications
    Suitable for: ICC, IHC-P, ELISA, WBmore details
  • Minimal
    cross-reactivity

    Cow, Horse, Human, Mouse, Rabbit, Sheep more details

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antiserum was cross adsorbed using bovine, horse, human, mouse, rat and sheep immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab102248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/200 - 1/2000.
IHC-P 1/200 - 1/2000.
ELISA 1/100 - 1/30000. Coating: Use 1/100 - 1/500 dilution; Primary: Use 1/1000 - 1/30000 dilution.
WB 1/1000 - 1/30000. Colorimetric: Use 1/1000 - 1/10000 dilution; Chemiluminescent: Use 1/1000 - 1/30000 dilution.

References

This product has been referenced in:
  • Jendresen C  et al. The Alzheimer's disease risk factors apolipoprotein E and TREM2 are linked in a receptor signaling pathway. J Neuroinflammation 14:59 (2017). Read more (PubMed: 28320424) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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