Overview

  • Product name
    Rabbit Anti-Rat IgG H&L (Texas Red ®)
    See all IgG secondary antibodies
  • Target species
    Rat
  • Tested applications
    Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details
  • Immunogen

    Rat IgG whole molecule

  • Conjugation
    Texas Red ®. Ex: 596nm, Em: 620nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Texas RedTM Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 4.9 moles Texas Redä per mole of Rabbit IgG
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunomicroscopy Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.
ICC/IF 1/1000 - 1/5000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ELISA 1/10000 - 1/50000.

Images

  • Immunofluorescence analysis of mouse fibroblasts, stained with a rat anti-mouse Lamp1 primary antibody and detected with ab6732 at 1/100 dilution.

References

This product has been referenced in:
  • Sun Y  et al. Ex Vivo and in Vivo Effects of Isofagomine on Acid ß-Glucosidase Variants and Substrate Levels in Gaucher Disease. J Biol Chem 287:4275-87 (2012). ICC/IF . Read more (PubMed: 22167193) »
  • Teranishi N  et al. Identification of neovasculature using nestin in colorectal cancer. Int J Oncol 30:593-603 (2007). Read more (PubMed: 17273760) »

See all 2 Publications for this product

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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