Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab8519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Dot blot
  • Application notesRecommended dilution as secondary antibody in ELISA is 1:1000.
    Recommended dilution as secondary antibody in dot-blotting is 1:100.
  • References for Rabbit Anti-Rat IgG+IgM+IgA H&L (ab8519)

    This product has been referenced in:
    • Lahoud MH  et al. Targeting antigen to mouse dendritic cells via Clec9A induces potent CD4 T cell responses biased toward a follicular helper phenotype. J Immunol 187:842-50 (2011). Read more (PubMed: 21677141) »

    See 1 Publication for this product

    Product Wall

    Thank you for contacting us.

    In general, the selection of the immunogen varies from antibody to antibody. Some antibodies are raised against synthetic peptides (based ona protein sequence which was derived originally from a DNA sequence), so...

    Read More

    I am sorry to hear that you have had problems with the 5 units of ab8519 that you purchased via VWR. the antibody has been tested in ELISA and Dot Blot only, not in an Ouchterlony gel. However I see no reason why the antibody should not work in this ap...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"