Rabbit Anti-Rat IgG+IgM+IgA H&L (FITC) (ab8520)

Overview

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, 0.1M Sodium chloride, 0.01M Sodium phosphate, pH 7.5
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesSpecific antibodies were absorpted by incubation with Sepharose-bound rat immunoglobulins. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized rat immunoglobulins a minimum of 65% protein bound.
  • Conjugation notesThe purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate.
  • Clonality Polyclonal
  • General notesF/P ratio is 3-5 mol Fluorescein per 1 mol of rabbit Ig. There is low background fluorescence of normal tissue in contrast to specific staining of sections treated with monoclonal rat anti-human keratin 18 antibodies
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab8520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Flow Cyt 1/40.
ICC/IF Use at an assay dependent concentration.

References for Rabbit Anti-Rat IgG+IgM+IgA H&L (FITC) (ab8520)

ab8520 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. I have contacted the originator of the antibody regarding your question and it is true, this antibody to rat immunoglobulin cross-reacts to mouse IgG. I have updated our datasheet to reflect this and I can certainly issu...

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Thank you for your enquiry. May I also take the time to thank you for filling in your questionnaire so comprehensively. I am sorry to hear that you have been obtaining high background with this antiserum. Given that you mention in your questionnaire...

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Yes, the Rabbit anti-Rat IgG,M,A recognizes the rat IgG2a and IgG2b equally.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"