Rabbit Anti-Sheep IgG Fc (ab102297)
Key features and details
- Rabbit Anti-Sheep IgG Fc
- Host species: Rabbit
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
Related conjugates and formulations
Overview
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Product name
Rabbit Anti-Sheep IgG Fc
See all IgG secondary antibodies -
Host species
Rabbit -
Target species
Sheep -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with sheep IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species. -
Tested applications
Suitable for: ICC, IHC-P, ELISA, WBmore details -
Immunogen
Other Immunogen Type corresponding to IgG.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antiserum was solid phase adsorbed to ensure class specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab102297 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
1/200 - 1/2000.
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IHC-P |
1/200 - 1/2000.
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ELISA |
1/100 - 1/30000. (Coating: 1/100 - 1/500; Primary: 1/1000 - 1/30000).
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WB |
1/1000 - 1/30000. (Colorimetric: 1/1000 - 1/10000; Chemiluminescent: 1/1000 - 1/30000).
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Notes |
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ICC
1/200 - 1/2000. |
IHC-P
1/200 - 1/2000. |
ELISA
1/100 - 1/30000. (Coating: 1/100 - 1/500; Primary: 1/1000 - 1/30000). |
WB
1/1000 - 1/30000. (Colorimetric: 1/1000 - 1/10000; Chemiluminescent: 1/1000 - 1/30000). |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (2)
ab102297 has been referenced in 2 publications.
- Lee YK et al. Carbohydrate Ligands for COVID-19 Spike Proteins. Viruses 14:N/A (2022). PubMed: 35215921
- Subharat S et al. Vaccination of Sheep with a Methanogen Protein Provides Insight into Levels of Antibody in Saliva Needed to Target Ruminal Methanogens. PLoS One 11:e0159861 (2016). ELISA ; Sheep . PubMed: 27472482