Rabbit Anti-Sheep IgG H&L (Alkaline Phosphatase) (ab6748)

Overview

  • Product nameRabbit Anti-Sheep IgG H&L (Alkaline Phosphatase)
    See all IgG secondary antibodies
  • Target speciesSheep
  • Tested applicationsSuitable for: IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-Frmore details
  • Immunogen

    Sheep IgG whole molecule

  • ConjugationAlkaline Phosphatase

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage bufferpH: 8
    Preservative: 0.1% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.788% Tris HCl, 0.0095% Magnesium chloride, 0.014% Zinc chloride, 50% Glycerol
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads
  • ClonalityPolyclonal
  • General notes

    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons).

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6748 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
WB
ELISA
Immunomicroscopy
Dot blot
ICC/IF
IHC-Fr
  • Application notessuitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency
  • References for Rabbit Anti-Sheep IgG H&L (Alkaline Phosphatase) (ab6748)

    This product has been referenced in:
    • Corti D  et al. Analysis of memory B cell responses and isolation of novel monoclonal antibodies with neutralizing breadth from HIV-1-infected individuals. PLoS One 5:e8805 (2010). ELISA ; Human . Read more (PubMed: 20098712) »
    • Richardson MR  et al. Diabetic dyslipidemia and exercise alter the plasma low-density lipoproteome in Yucatan pigs. Proteomics 9:2468-83 (2009). WB . Read more (PubMed: 19402046) »

    See all 2 Publications for this product

    Product Wall

    Thank you for your enquiry. My colleague Dr Sarah Mardle is out of office until Thursday morning. As soon as she gets back, she will get in touch with you and deal with your enquiry. Thank you for your patience.

    I'm very sorry to hear you are having problems with ab6748. We take your complaint very seriously and would appreciate if you could please clarify whether you have tested the antibody with no blocking at all and tell me what permeabilising agent has be...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"