Rabbit Anti-Sheep IgG H&L (Alkaline Phosphatase) preadsorbed (ab7112)

Overview

  • Product nameRabbit Anti-Sheep IgG H&L (Alkaline Phosphatase) preadsorbed
    See all IgG secondary antibodies
  • Target speciesSheep
  • Tested applicationsSuitable for: ICC/IF, IHC-P, IHC-Fr, WB, ELISA, Immunomicroscopy, Dot blotmore details
  • Minimal
    cross-reactivity

    Human more details
  • Immunogen

    Full length native Sheep IgG (purified).

  • ConjugationAlkaline Phosphatase

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 50% Glycerol, 10mg/ml BSA, 0.15M Sodium chloride, 0.05M Tris HCl, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 8.0
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesAlkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
  • Conjugation notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads
  • ClonalityPolyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200 - 1/1000.
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
WB 1/400 - 1/1600.
ELISA 1/1000 - 1/5000.
Immunomicroscopy Use at an assay dependent dilution.
Dot blot Use at an assay dependent dilution.

References for Rabbit Anti-Sheep IgG H&L (Alkaline Phosphatase) preadsorbed (ab7112)

ab7112 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"