Rabbit Anti-Sheep IgG H&L (Alkaline Phosphatase) preadsorbed (ab7112)

Overview

  • Product name
    Rabbit Anti-Sheep IgG H&L (Alkaline Phosphatase) preadsorbed
    See all IgG secondary antibodies
  • Target species
    Sheep
  • Tested applications
    Suitable for: ICC/IF, IHC-P, IHC-Fr, WB, ELISA, Immunomicroscopy, Dot blotmore details
  • Minimal
    cross-reactivity

    Human more details
  • Immunogen

    Full length native Sheep IgG (purified).

  • Conjugation
    Alkaline Phosphatase

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 50% Glycerol, 10mg/ml BSA, 0.15M Sodium chloride, 0.05M Tris HCl, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 8.0
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
  • Conjugation notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200 - 1/1000.
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
WB 1/400 - 1/1600.
ELISA 1/1000 - 1/5000.
Immunomicroscopy Use at an assay dependent dilution.
Dot blot Use at an assay dependent dilution.

References

ab7112 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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