Rabbit Anti-Sheep IgG H&L (Alkaline Phosphatase) preadsorbed (ab7112)
Key features and details
- Rabbit Anti-Sheep IgG H&L (Alkaline Phosphatase) preadsorbed
- Conjugation: Alkaline Phosphatase
- Host species: Rabbit
- Suitable for: ICC/IF, IHC-P, IHC-Fr, WB, ELISA, Immunomicroscopy, Dot blot
Overview
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Product name
Rabbit Anti-Sheep IgG H&L (Alkaline Phosphatase) preadsorbed
See all IgG secondary antibodies -
Host species
Rabbit -
Target species
Sheep -
Tested applications
Suitable for: ICC/IF, IHC-P, IHC-Fr, WB, ELISA, Immunomicroscopy, Dot blotmore details -
Minimal
cross-reactivity
Human more details -
Immunogen
Full length native Sheep IgG (purified).
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Conjugation
Alkaline Phosphatase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.00136% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons) -
Conjugation notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads -
Clonality
Polyclonal -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/200 - 1/1000.
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IHC-P |
Use at an assay dependent dilution.
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|
IHC-Fr |
Use at an assay dependent dilution.
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|
WB |
1/400 - 1/1600.
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|
ELISA |
1/1000 - 1/5000.
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Immunomicroscopy |
Use at an assay dependent dilution.
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|
Dot blot |
Use at an assay dependent dilution.
|
Notes |
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ICC/IF
1/200 - 1/1000. |
IHC-P
Use at an assay dependent dilution. |
IHC-Fr
Use at an assay dependent dilution. |
WB
1/400 - 1/1600. |
ELISA
1/1000 - 1/5000. |
Immunomicroscopy
Use at an assay dependent dilution. |
Dot blot
Use at an assay dependent dilution. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab7112 has not yet been referenced specifically in any publications.