Overview

  • Product nameRabbit Anti-Sheep IgG H&L (Biotin)
    See all IgG secondary antibodies
  • Target speciesSheep
  • Tested applicationsWB, ELISA, Immunomicroscopy, Dot Blot, ICC/IF, IHC-P, IHC-Frmore details
  • Immunogen

    Sheep IgG whole molecule

  • ConjugationBiotin

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesBiotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule
  • Conjugation notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab6746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000.
ELISA 1/140000.
Immunomicroscopy Use at an assay dependent concentration.
Dot Blot Use at an assay dependent concentration.
ICC/IF 1/500 - 1/3000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Rabbit Anti-Sheep IgG H&L (Biotin) images

  • Ab6746 was used at dilution 1/500 with the primary antibody ab9013 in IHC-P. See the review on ab9013.

References for Rabbit Anti-Sheep IgG H&L (Biotin) (ab6746)

This product has been referenced in:
  • Thibault K  et al. BDNF-dependent plasticity induced by peripheral inflammation in the primary sensory and the cingulate cortex triggers cold allodynia and reveals a major role for endogenous BDNF as a tuner of the affective aspect of pain. J Neurosci 34:14739-51 (2014). IHC ; Rat . Read more (PubMed: 25355226) »
  • Periz J  et al. Rotavirus mRNAS are released by transcript-specific channels in the double-layered viral capsid. Proc Natl Acad Sci U S A 110:12042-7 (2013). Read more (PubMed: 23818620) »

See all 3 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab6746.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"