Overview

  • Product nameRabbit Anti-Sheep IgG H&L (FITC)
    See all IgG secondary antibodies
  • Target speciesSheep
  • Tested applicationsSuitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details
  • Immunogen

    Sheep whole molecule

  • ConjugationFITC. Ex: 493nm, Em: 528nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferpH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • ClonalityPolyclonal
  • General notes

    Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunomicroscopy Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.
ICC/IF 1/1000 - 1/5000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ELISA 1/10000 - 1/50000.

References for Rabbit Anti-Sheep IgG H&L (FITC) (ab6743)

This product has been referenced in:
  • Wilding C  et al. ?-Synuclein antibodies have neuroprotective potential on neuroretinal cells via proteins of the mitochondrial apoptosis pathway. PLoS One 9:e90737 (2014). Read more (PubMed: 24595072) »
  • Nguyen MD  et al. Cardiac cell culture model as a left ventricle mimic for cardiac tissue generation. Anal Chem 85:8773-9 (2013). Read more (PubMed: 23952579) »

See all 7 Publications for this product

Product Wall

For IHC-P

Excellent
Abreviews
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
I tried 1:100 and 1:500 dilutions for this antibody. 1:500 works well with an hour incubation at room temperature.
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Abcam user community

Verified customer

Submitted Aug 20 2014

Thank you for your inquiry.

Unfortunately, we do not determine the exact molecular weight for our antibodies.

http://www.abcam.com/ab6743: asshown on the datasheet,the fluorochrome/Protein Ratio is2.3 moles DTAF per mole of Rabbit IgG...

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Gracias por tu respuesta.

No tenemos un protocolo concreto para el uso de este anticuerpo mediante immunohistoquímica, sino protocolos generales que pueden servir de guía para los distintos ensayos.

Te adjunto el link ...

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Gracias por tu respuesta.
Te confirmo que ab53435 solo se ha testado en secciones congeladas. No tenemos, pues, ninguna evidencia de que vaya a detectar el epítopo en secciones incluidas en parafina.
Así pues, puedes optar por testarlo en est...

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We have no experience using this secondary antibody in this technique, so I am afraid that I cannot offer any specific advice. However, this Rabbit polyclonal to sheep IgG will certainly work in conjunction with a sheep primary.

Antibodies are usually stable in buffers containing triton-X. I've personally used antibodies in such buffers with no problems. Also, several automated IHC staining systems routinely use such surfactants in their buffer solutions. We have never actuall...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"